haha100_v_medium

CultureMech:001042 bacterial COMPLEX LIQUID
Original name: HAHA100 V MEDIUM

Overview

External ontology
mediadive.medium:1566 (source link unavailable) — HAHA100 V MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1566.pdf

Composition

flowchart LR
    CultureMech_001042["haha100_v_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001042 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001042 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001042 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001042 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_001042 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_KBr["KBr"]
    CultureMech_001042 --> ing5_KBr
    CHEBI_32030["CHEBI:32030"]
    ing5_KBr -.-> CHEBI_32030
    ing6_NH4Cl["NH4Cl"]
    CultureMech_001042 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_KH2PO4["KH2PO4"]
    CultureMech_001042 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_H3BO3["H3BO3"]
    CultureMech_001042 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_SrCl2["SrCl2"]
    CultureMech_001042 --> ing9_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing9_SrCl2 -.-> CHEBI_36383
    ing10_NaF["NaF"]
    CultureMech_001042 --> ing10_NaF
    CHEBI_28741["CHEBI:28741"]
    ing10_NaF -.-> CHEBI_28741
    ing11_Tryptone_peptone["Tryptone peptone"]
    CultureMech_001042 --> ing11_Tryptone_peptone
    ing12_Casamino_acids["Casamino acids"]
    CultureMech_001042 --> ing12_Casamino_acids
    ing13_Yeast_extract["Yeast extract"]
    CultureMech_001042 --> ing13_Yeast_extract
    ing14___D_Glucose_monohydrate["α-D-Glucose monohydrate"]
    CultureMech_001042 --> ing14___D_Glucose_monohydrate
    ing15_Cellobiose["Cellobiose"]
    CultureMech_001042 --> ing15_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing15_Cellobiose -.-> CHEBI_17057
    ing16_NaHCO3["NaHCO3"]
    CultureMech_001042 --> ing16_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing16_NaHCO3 -.-> CHEBI_32139
    ing17_NaOH["NaOH"]
    CultureMech_001042 --> ing17_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing17_NaOH -.-> CHEBI_32145
    ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001042 --> ing18_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001042 --> ing19_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing20_Riboflavin["Riboflavin"]
    CultureMech_001042 --> ing20_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing20_Riboflavin -.-> CHEBI_17015
    ing21_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001042 --> ing21_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing21_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001042 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001042 --> ing23_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing24_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001042 --> ing24_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing24_Calcium_pantothenate -.-> CHEBI_31345
    ing25_Biotin["Biotin"]
    CultureMech_001042 --> ing25_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing25_Biotin -.-> CHEBI_15956
    ing26_Folic_acid["Folic acid"]
    CultureMech_001042 --> ing26_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing26_Folic_acid -.-> CHEBI_27470
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_001042 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_Na2_EDTA["Na2-EDTA"]
    CultureMech_001042 --> ing28_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing28_Na2_EDTA -.-> CHEBI_64734
    ing29_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001042 --> ing29_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing29_FeCl2_x_4_H2O -.-> CHEBI_86249
    CultureMech_001042 --> more["...6 more"]

Ingredients (36)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
26.37 G_PER_L CHEBI:26710
sodium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.8 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.67 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.47 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 KCl
Cl.K 74.551 g/mol
0.72 G_PER_L CHEBI:32588
potassium chloride
6 KBr
Br.K 119.002 g/mol
0.1 G_PER_L CHEBI:32030
potassium bromide
7 NH4Cl
Cl.H4N 53.492 g/mol
0.8 MG_PER_L CHEBI:31206
ammonium chloride
8 KH2PO4
H2O4P.K 136.084 g/mol
0.02 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
9 H3BO3
H3BO3 61.833 g/mol
0.082 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.02, 0.062]
10 SrCl2
2Cl.Sr 158.526 g/mol
0.02 G_PER_L CHEBI:36383
strontium dichloride
11 NaF
F.Na 41.988 g/mol
3 MG_PER_L CHEBI:28741
sodium fluoride
12 Tryptone peptone 0.1 G_PER_L
13 Casamino acids 0.1 G_PER_L
14 Yeast extract 0.1 G_PER_L
15 α-D-Glucose monohydrate 1 G_PER_L
16 Cellobiose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:17057
cellobiose
17 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
18 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
19 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
20 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
21 Riboflavin
C17H20N4O6 376.369 g/mol
0.1 G_PER_L CHEBI:17015
riboflavin
22 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.5 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
23 Nicotinic acid
C6H5NO2 123.111 g/mol
0.5 G_PER_L CHEBI:15940
nicotinic acid
24 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.5 G_PER_L CHEBI:30961
pyridoxine hydrochloride
25 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.5 G_PER_L CHEBI:31345
Calcium pantothenate
26 Biotin
C10H16N2O3S 244.316 g/mol
0.001 G_PER_L CHEBI:15956
biotin
27 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
28 Vitamin B12 0.01 G_PER_L CHEBI:176843
vitamin B12
29 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
5.2 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
30 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
31 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
32 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
33 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
34 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.017 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
35 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
36 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 7.5 with 1 M HCl or 1 M NaHCO3
  2. Autoclave at 121 °C for 20 min.
  3. Before inoculation add from sterile stock solutions:
  4. Filter sterilize (0.2 µm pore-size) and store at 4°C.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.856218Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1566
2026-01-28T19:48:28.412790Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:17.696623Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:00.686170Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001042
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.319473+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HAHA100 V MEDIUM" to "haha100_v_medium" for consistent matching
2026-06-05T07:41:06.985363+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.419384+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:59.255044+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:36.788539+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint dfc077f3796920b2ba6dfbfff95755e7a6d3f392b398c2dc9296e7e0d38b4125