Overview
- External ontology
- mediadive.medium:1571 (source link unavailable) — MICROBACTER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1571.pdf
Composition
flowchart LR
CultureMech_001048["microbacter_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001048 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001048 --> ing1_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing1_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing2_NH4Cl["NH4Cl"]
CultureMech_001048 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_001048 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001048 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001048 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Yeast_extract["Yeast extract"]
CultureMech_001048 --> ing6_Yeast_extract
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001048 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
ing8_Resazurin["Resazurin"]
CultureMech_001048 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_L_Cysteine["L-Cysteine"]
CultureMech_001048 --> ing9_L_Cysteine
CHEBI_17561["CHEBI:17561"]
ing9_L_Cysteine -.-> CHEBI_17561
ing10_HCl["HCl"]
CultureMech_001048 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001048 --> ing11_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
ing12_ZnCl2["ZnCl2"]
CultureMech_001048 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001048 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_001048 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001048 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001048 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001048 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001048 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_Biotin["Biotin"]
CultureMech_001048 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_Folic_acid["Folic acid"]
CultureMech_001048 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001048 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001048 --> ing22_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing22_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing23_Riboflavin["Riboflavin"]
CultureMech_001048 --> ing23_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing23_Riboflavin -.-> CHEBI_17015
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_001048 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_001048 --> ing25_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing25_D_Calcium_pantothenate -.-> CHEBI_31345
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_001048 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001048 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28_Lipoic_acid["Lipoic acid"]
CultureMech_001048 --> ing28_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28_Lipoic_acid -.-> CHEBI_16494
ing29_NaOH["NaOH"]
CultureMech_001048 --> ing29_NaOH
CHEBI_32145["CHEBI:32145"]
ing29_NaOH -.-> CHEBI_32145
CultureMech_001048 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.41 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.53 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.3 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 | Yeast extract | 2 G_PER_L | ||
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.36 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
L-Cysteine
C3H7NO2S 121.161 g/mol
|
0.5 G_PER_L |
CHEBI:17561
L-cysteine
|
|
| 11 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 12 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 23 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 24 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 26 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 27 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 29 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 30 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 31 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 32 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve in 1 liter, boil under N2, add 0.5 g of L-cysteine and adjust pH to 6.4 (should be pH 5.5 after autoclaving). Disperse in tubes and bottles (leave 50% of container volume as gas phase). Change to gas mixture N2/CO2; 80/20%) and autoclave for 20 minutes at 121°C.
- To 100 ml sterile medium add aseptically
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- microbacter_medium (CultureMech:001048)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.878126Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1571 |
| 2026-01-28T19:48:19.031166Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:00.755748Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001048 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.395179+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MICROBACTER MEDIUM" to "microbacter_medium" for consistent matching |
| 2026-06-05T07:41:33.802388+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.802779+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:45.355607+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6c3022d52604d7ca55e369342813a6765648004191cb8db91d3cbeab3c8951d7 |