hmd_medium

CultureMech:001049 bacterial COMPLEX SOLID_AGAR
Original name: HMD-MEDIUM

Overview

External ontology
mediadive.medium:1572 (source link unavailable) — HMD-MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.5}
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1572.pdf

Composition

flowchart LR
    CultureMech_001049["hmd_medium"]
    ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001049 --> ing0_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing1_K2SO4["K2SO4"]
    CultureMech_001049 --> ing1_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing1_K2SO4 -.-> CHEBI_32036
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001049 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_001049 --> ing3_Yeast_extract
    ing4_Casamino_acids["Casamino acids"]
    CultureMech_001049 --> ing4_Casamino_acids
    ing5_NaCl["NaCl"]
    CultureMech_001049 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Agar["Agar"]
    CultureMech_001049 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
20 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
2 K2SO4
2K.O4S 174.259 g/mol
5 G_PER_L CHEBI:32036
potassium sulfate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 Yeast extract 0.1 G_PER_L
5 Casamino acids 0.5 G_PER_L
6 NaCl
Cl.Na 58.443 g/mol
250 G_PER_L CHEBI:26710
sodium chloride
7 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.0 - 7.5

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.883421Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1572
2026-01-28T19:48:33.862147Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:00.763622Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001049
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.404295+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HMD-MEDIUM" to "hmd_medium" for consistent matching
2026-05-20T06:00:47.081582+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.5' -> {'min': 7.0, 'max': 7.5}
2026-06-05T07:40:16.074923+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.207443+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:42.612479+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:34.518910+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 8a755fbc7e646dda1a20b64ece0dee3f681165f55ed80bbd4aeb3843e6969b82