Overview
- External ontology
- mediadive.medium:1579 (source link unavailable) — PYROBACULUM FERRIREDUCENS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.5, 'max': 6.8}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1579.pdf
Composition
flowchart LR
CultureMech_001053["pyrobaculum_ferrireducens_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001053 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KCl["KCl"]
CultureMech_001053 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_KH2PO4["KH2PO4"]
CultureMech_001053 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001053 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001053 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Yeast_extract["Yeast extract"]
CultureMech_001053 --> ing5_Yeast_extract
ing6_KNO3["KNO3"]
CultureMech_001053 --> ing6_KNO3
CHEBI_63043["CHEBI:63043"]
ing6_KNO3 -.-> CHEBI_63043
ing7_NaHCO3["NaHCO3"]
CultureMech_001053 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_HCl["HCl"]
CultureMech_001053 --> ing8_HCl
CHEBI_17883["CHEBI:17883"]
ing8_HCl -.-> CHEBI_17883
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001053 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_ZnCl2["ZnCl2"]
CultureMech_001053 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001053 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_001053 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001053 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001053 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001053 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001053 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_NaOH["NaOH"]
CultureMech_001053 --> ing17_NaOH
CHEBI_32145["CHEBI:32145"]
ing17_NaOH -.-> CHEBI_32145
ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001053 --> ing18_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001053 --> ing19_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing20_Biotin["Biotin"]
CultureMech_001053 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_Folic_acid["Folic acid"]
CultureMech_001053 --> ing21_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing21_Folic_acid -.-> CHEBI_27470
ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001053 --> ing22_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing23_Thiamine_HCl["Thiamine HCl"]
CultureMech_001053 --> ing23_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing23_Thiamine_HCl -.-> CHEBI_49105
ing24_Riboflavin["Riboflavin"]
CultureMech_001053 --> ing24_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing24_Riboflavin -.-> CHEBI_17015
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_001053 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001053 --> ing26_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_D_____pantothenate -.-> CHEBI_31345
ing27_Vitamin_B12["Vitamin B12"]
CultureMech_001053 --> ing27_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing27_Vitamin_B12 -.-> CHEBI_176843
ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001053 --> ing28_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
ing29__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001053 --> ing29__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing29__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.329013 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.329013 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.329013 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.329013 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.329013 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Yeast extract | 0.997009 G_PER_L | ||
| 7 |
KNO3
K.NO3 101.102 g/mol
|
0.997009 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.99402 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 19 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 20 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 22 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 23 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 24 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 25 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 29 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 30 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except yeast extract, nitrate, bicarbonate and vitamins) and sparge medium with 100% CO2 gas for at least 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add yeast extract, nitrate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be adjusted to 6.5 - 6.8, if necessary.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pyrobaculum_ferrireducens_medium (CultureMech:001053)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.895240Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1579 |
| 2026-02-06T05:10:35.316377Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:00.801268Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001053 |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.445621+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYROBACULUM FERRIREDUCENS MEDIUM" to "pyrobaculum_ferrireducens_medium" for consistent matching |
| 2026-05-20T06:02:03.424121+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.5-6.8' -> {'min': 6.5, 'max': 6.8} |
| 2026-06-05T07:41:43.236327+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.775930+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:10.640829+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.368610+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9f82836846bd70766cae3a18f12563a2f1611d10c449b9369768b0e9702b91d6 |