Overview
- External ontology
- mediadive.medium:1580 (source link unavailable) — MODIFIED EGGERTH-GAGNON MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1580.pdf Commercial Product: MacConkey Agar Supplier: Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) Catalog: BD 212123, Sigma 05465 Source: https://microbenotes.com/macconkey-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001055["modified_eggerth_gagnon_medium"]
ing0_Peptone["Peptone"]
CultureMech_001055 --> ing0_Peptone
ing1_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001055 --> ing1_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing1_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing2_Peptone["Peptone"]
CultureMech_001055 --> ing2_Peptone
ing3_Proteose_peptone["Proteose peptone"]
CultureMech_001055 --> ing3_Proteose_peptone
ing4_Lactose_monohydrate["Lactose monohydrate"]
CultureMech_001055 --> ing4_Lactose_monohydrate
CHEBI_17716["CHEBI:17716"]
ing4_Lactose_monohydrate -.-> CHEBI_17716
ing5_Bile_salts["Bile salts"]
CultureMech_001055 --> ing5_Bile_salts
CHEBI_22868["CHEBI:22868"]
ing5_Bile_salts -.-> CHEBI_22868
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_001055 --> ing6_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing6_Sodium_chloride -.-> CHEBI_26710
ing7_Neutral_red["Neutral red"]
CultureMech_001055 --> ing7_Neutral_red
CHEBI_86370["CHEBI:86370"]
ing7_Neutral_red -.-> CHEBI_86370
ing8_Crystal_violet["Crystal violet"]
CultureMech_001055 --> ing8_Crystal_violet
CHEBI_41688["CHEBI:41688"]
ing8_Crystal_violet -.-> CHEBI_41688
ing9_Agar["Agar"]
CultureMech_001055 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_Agar["Agar"]
CultureMech_001055 --> ing10_Agar
ing10_Agar -.-> CHEBI_2509
ing11_Sheep_blood["Sheep blood"]
CultureMech_001055 --> ing11_Sheep_blood
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 10 G_PER_L | ||
| 2 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
4 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 3 |
Peptone
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
17.0 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
Pancreatic digest of gelatin, provides nitrogen and amino acids; Constituent of MacConkey Agar commercial product |
| 4 |
Proteose peptone
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
3.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Meat and casein digest, provides additional nutrients; Constituent of MacConkey Agar commercial product |
| 5 |
Lactose monohydrate
C12H22O11 342.297 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
10.0 G_PER_L |
CHEBI:17716
lactose
|
Fermentable carbohydrate for differentiation. Fermentation produces acid causing pH drop and color change; Constituent of MacConkey Agar commercial product |
| 6 |
Bile salts
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
1.5 G_PER_L |
CHEBI:22868
bile salt
|
Selective agent - inhibits gram-positive bacteria; Constituent of MacConkey Agar commercial product |
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of MacConkey Agar commercial product |
| 8 |
Neutral red
C15H16N4.HCl 288.782 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
0.03 G_PER_L |
CHEBI:86370
neutral red
|
pH indicator - turns red/pink below pH 6.8, absorbed by colonies of lactose fermenters; Constituent of MacConkey Agar commercial product |
| 9 |
Crystal violet
C25H30N3.Cl 407.989 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
0.001 G_PER_L |
CHEBI:41688
crystal violet
|
Selective agent - inhibits gram-positive bacteria; Constituent of MacConkey Agar commercial product |
| 10 |
Agar
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
13.5 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent; Constituent of MacConkey Agar commercial product |
| 11 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 12 | Sheep blood | 50 G_PER_L |
Preparation
- Autoclave at 121°C, let agar cool to 50°C and aseptically add 50 ml sheep blood.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_eggerth_gagnon_medium (CultureMech:001055)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.899748Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1580 |
| 2026-03-13T06:19:00.813282Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001055 |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.459349+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED EGGERTH-GAGNON MEDIUM" to "modified_eggerth_gagnon_medium" for consistent matching |
| 2026-05-17T07:07:31.197980+00:00 | cleanup_residual_errors.py | CLEANUP_RESIDUAL_ERRORS | R01_flatten_synonym_text=2 |
| 2026-06-05T07:41:36.573058+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:48.262312+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3f7985dccd6ab4bb1bf672feaac9a842b884af19a033e9d9a7d2349ef784f815 |