medium_for_ammonia_oxidizing_bacteria_strains_from_soil

CultureMech:001058 bacterial DEFINED LIQUID
Original name: MEDIUM FOR AMMONIA OXIDIZING BACTERIA (STRAINS FROM SOIL)

Overview

External ontology
mediadive.medium:1583 (source link unavailable) — MEDIUM FOR AMMONIA OXIDIZING BACTERIA (STRAINS FROM SOIL)
Applications
  • Microbial cultivation
pH
7.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1583.pdf

Composition

flowchart LR
    CultureMech_001058["medium_for_ammonia_oxidizing_bacteria_strains_from_soil"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_001058 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001058 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_KCl["KCl"]
    CultureMech_001058 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001058 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001058 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NaCl["NaCl"]
    CultureMech_001058 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_HCl["HCl"]
    CultureMech_001058 --> ing6_HCl
    CHEBI_17883["CHEBI:17883"]
    ing6_HCl -.-> CHEBI_17883
    ing7_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_001058 --> ing7_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing7_MnSO4_x_4_H2O -.-> CHEBI_86358
    ing8_H3BO3["H3BO3"]
    CultureMech_001058 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001058 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001058 --> ing10__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing10__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001058 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001058 --> ing12_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing13_Cresol_red["Cresol red"]
    CultureMech_001058 --> ing13_Cresol_red
    CHEBI_86218["CHEBI:86218"]
    ing13_Cresol_red -.-> CHEBI_86218

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.535 G_PER_L CHEBI:31206
ammonium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.054 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 KCl
Cl.K 74.551 g/mol
0.074 G_PER_L CHEBI:32588
potassium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.049 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.147 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 NaCl
Cl.Na 58.443 g/mol
0.584 G_PER_L CHEBI:26710
sodium chloride
7 HCl
HCl 36.461 g/mol
25 G_PER_L CHEBI:17883
hydrogen chloride
 (1M)
8 MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
0.045 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
9 H3BO3
H3BO3 61.833 g/mol
0.049 G_PER_L CHEBI:33118
boric acid
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.043 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.037 G_PER_L CHEBI:91249
ammonium molybdate
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.973 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.025 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
14 Cresol red
C21H18O5S 382.437 g/mol
0.5 G_PER_L CHEBI:86218
cresol red

Preparation

  1. Autoclave, subsequently adjust pH of the medium to about 7.8 by addition of sterile 10% NaHCO3 (medium is turning from yellow to pink).
  2. Important: During growth, add NaHCO3 to readjust pH as soon as pH indicator changes to yellow. For stock cultures, 5 g l-1 CaCO3 (will stay as a precipitate) or 4.8 g l-1 HEPES may be added to keep pH neutral for a longer time.
  3. Aeration must be adjusted according to the density and activity of the culture. At low cell concentrations (which is the case most of the time) cells are sensitive to strong aeration (don't shake the cultures).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.904816Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1583
2026-03-13T06:19:00.828455Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001058
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.475917+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR AMMONIA OXIDIZING BACTERIA (STRAINS FROM SOIL)" to "medium_for_ammonia_oxidizing_bacteria_strains_from_soil" for consistent matching
2026-06-05T07:41:28.846821+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:05.975645+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:43.014529+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint a3093a30b0a478b40782f55db2e4e8c2ef423296eddeca1b78f855de3ce000ad