cyanobacteria_medium_bg11_tes_buffered

CultureMech:001071 bacterial COMPLEX LIQUID
Original name: Cyanobacteria Medium BG11+ (TES buffered)

Overview

External ontology
mediadive.medium:1593a (source link unavailable) — Cyanobacteria Medium BG11+ (TES buffered)
Applications
  • Microbial cultivation
pH
8.0
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001071["cyanobacteria_medium_bg11_tes_buffered"]
    ing0_TES["TES"]
    CultureMech_001071 --> ing0_TES
    CHEBI_39035["CHEBI:39035"]
    ing0_TES -.-> CHEBI_39035
    ing1_NaNO3["NaNO3"]
    CultureMech_001071 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_Mg_SO4__x_7_H2O["Mg(SO4) x 7 H2O"]
    CultureMech_001071 --> ing2_Mg_SO4__x_7_H2O
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001071 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Na3_citrate_x_2_H2O["Na3-citrate x 2 H2O"]
    CultureMech_001071 --> ing4_Na3_citrate_x_2_H2O
    CHEBI_32142["CHEBI:32142"]
    ing4_Na3_citrate_x_2_H2O -.-> CHEBI_32142
    ing5_Na_EDTA_x_2_H2O["Na-EDTA x 2 H2O"]
    CultureMech_001071 --> ing5_Na_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing5_Na_EDTA_x_2_H2O -.-> CHEBI_64758
    ing6_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001071 --> ing6_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing7_Na2CO3["Na2CO3"]
    CultureMech_001071 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_Fe_NH4_citrate["Fe(NH4)citrate"]
    CultureMech_001071 --> ing8_Fe_NH4_citrate
    CHEBI_31604["CHEBI:31604"]
    ing8_Fe_NH4_citrate -.-> CHEBI_31604
    ing9_Vitamin_B12["Vitamin B12"]
    CultureMech_001071 --> ing9_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing9_Vitamin_B12 -.-> CHEBI_176843
    ing10_H3BO3["H3BO3"]
    CultureMech_001071 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001071 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001071 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001071 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001071 --> ing14_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing15_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001071 --> ing15_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing15_Co_NO3_2_x_6_H2O -.-> CHEBI_86214

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 TES 2.3 G_PER_L CHEBI:39035
TES
2 NaNO3
NO3.Na 84.994 g/mol
5 G_PER_L CHEBI:63005
sodium nitrate
3 Mg(SO4) x 7 H2O 10 G_PER_L
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
10 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 Na3-citrate x 2 H2O
C6H5O7.2H2O.3Na 294.099 g/mol
10 G_PER_L CHEBI:32142
sodium citrate dihydrate
6 Na-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
10 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
7 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 Na2CO3
CO3.2Na 105.988 g/mol
2 G_PER_L CHEBI:29377
sodium carbonate
9 Fe(NH4)citrate 1 G_PER_L CHEBI:31604
ferric ammonium citrate
10 Vitamin B12 1 G_PER_L CHEBI:176843
vitamin B12
11 H3BO3
H3BO3 61.833 g/mol
2.9 G_PER_L CHEBI:33118
boric acid
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.81 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.22 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.39 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
10 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
16 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
10 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate

Preparation

  1. Adjust pH to 8.0.
  2. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
  3. Autoclave at 121 °C for 20 min.
  4. After Cooling, add the following filter sterilized components:
  5. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.939381Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1593a
2026-01-28T19:48:07.146361Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:00.932958Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001071
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.592217+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Cyanobacteria Medium BG11+ (TES buffered)" to "cyanobacteria_medium_bg11_tes_buffered" for consistent matching
2026-06-05T07:40:55.112231+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.072688+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:29.442578+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 06f794821b0f0a141b0aa353c532d3642c03f2d0b8ce67eb1f4be460c912e1e9