francisella_persica_medium

CultureMech:001081 bacterial COMPLEX LIQUID
Original name: FRANCISELLA PERSICA MEDIUM

Overview

External ontology
mediadive.medium:1600 (source link unavailable) — FRANCISELLA PERSICA MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1600.pdf

Composition

flowchart LR
    CultureMech_001081["francisella_persica_medium"]
    ing0_L_15_powder["L-15 powder"]
    CultureMech_001081 --> ing0_L_15_powder
    ing1_DL_aspartic_acid["DL-aspartic acid"]
    CultureMech_001081 --> ing1_DL_aspartic_acid
    CHEBI_22660["CHEBI:22660"]
    ing1_DL_aspartic_acid -.-> CHEBI_22660
    ing2_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_001081 --> ing2_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing2_L_Glutamic_acid -.-> CHEBI_16015
    ing3_L_Glutamine["L-Glutamine"]
    CultureMech_001081 --> ing3_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing3_L_Glutamine -.-> CHEBI_18050
    ing4_L_Proline["L-Proline"]
    CultureMech_001081 --> ing4_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing4_L_Proline -.-> CHEBI_17203
    ing5_alpha_ketoglutaric_acid["alpha-ketoglutaric acid"]
    CultureMech_001081 --> ing5_alpha_ketoglutaric_acid
    CHEBI_30915["CHEBI:30915"]
    ing5_alpha_ketoglutaric_acid -.-> CHEBI_30915
    ing6_D_Glucose["D-Glucose"]
    CultureMech_001081 --> ing6_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing6_D_Glucose -.-> CHEBI_17634
    ing7_Demineralized_water["Demineralized water"]
    CultureMech_001081 --> ing7_Demineralized_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Demineralized_water -.-> CHEBI_15377
    ing8_Tryptose_phosphate["Tryptose-phosphate"]
    CultureMech_001081 --> ing8_Tryptose_phosphate
    ing9_FBS["FBS"]
    CultureMech_001081 --> ing9_FBS
    ing10_cholesterol_lipid_concentrate["cholesterol lipid concentrate"]
    CultureMech_001081 --> ing10_cholesterol_lipid_concentrate
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001081 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_MnSO4_x_1_H2O["MnSO4 x 1 H2O"]
    CultureMech_001081 --> ing12_MnSO4_x_1_H2O
    CHEBI_86360["CHEBI:86360"]
    ing12_MnSO4_x_1_H2O -.-> CHEBI_86360
    ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001081 --> ing13_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001081 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_001081 --> ing15_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_Na2SeO3["Na2SeO3"]
    CultureMech_001081 --> ing16_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing16_Na2SeO3 -.-> CHEBI_48843
    ing17_Glutathione["Glutathione"]
    CultureMech_001081 --> ing17_Glutathione
    CHEBI_16856["CHEBI:16856"]
    ing17_Glutathione -.-> CHEBI_16856
    ing18_Ascorbic_acid["Ascorbic acid"]
    CultureMech_001081 --> ing18_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing18_Ascorbic_acid -.-> CHEBI_22652
    ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001081 --> ing19_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing20_Para_aminobenzoic_acid["Para-aminobenzoic acid"]
    CultureMech_001081 --> ing20_Para_aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing20_Para_aminobenzoic_acid -.-> CHEBI_30753
    ing21_Vitamin_B12["Vitamin B12"]
    CultureMech_001081 --> ing21_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing21_Vitamin_B12 -.-> CHEBI_176843
    ing22_D_Biotin["D-Biotin"]
    CultureMech_001081 --> ing22_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_D_Biotin -.-> CHEBI_15956

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 L-15 powder 1 G_PER_L
2 DL-aspartic acid
C4H7NO4 133.103 g/mol
0.835196 G_PER_L CHEBI:22660
aspartic acid
3 L-Glutamic acid
C5H9NO4 147.13 g/mol
1.39665 G_PER_L CHEBI:16015
L-glutamic acid
4 L-Glutamine
C5H10N2O3 146.146 g/mol
0.29228 G_PER_L CHEBI:18050
L-glutamine
5 L-Proline
C5H9NO2 115.132 g/mol
0.837989 G_PER_L CHEBI:17203
L-proline
6 alpha-ketoglutaric acid
C5H6O5 146.098 g/mol
0.835196 G_PER_L CHEBI:30915
2-oxoglutaric acid
7 D-Glucose
C6H12O6 180.156 g/mol
6.25419 G_PER_L CHEBI:17634
D-glucose
8 Demineralized water
H2O 18.015 g/mol
371.0 G_PER_L CHEBI:15377
water
 [Merged 2 duplicates: 346.0, 25.0]
9 Tryptose-phosphate 5 G_PER_L
10 FBS 2.5 G_PER_L
11 cholesterol lipid concentrate 200 G_PER_L
12 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
20 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
13 MnSO4 x 1 H2O
Mn.O4S 151.001 g/mol
16 G_PER_L CHEBI:86360
manganese(II) sulfate
14 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
2 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 Na2SeO3
2Na.O3Se 172.937 g/mol
2 G_PER_L CHEBI:48843
disodium selenite
18 Glutathione
C10H17N3O6S 307.328 g/mol
100 G_PER_L CHEBI:16856
glutathione
19 Ascorbic acid 100 G_PER_L CHEBI:22652
ascorbic acid
20 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
5 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
21 Para-aminobenzoic acid
C7H7NO2 137.138 g/mol
10 G_PER_L CHEBI:30753
4-aminobenzoic acid
22 Vitamin B12 5 G_PER_L CHEBI:176843
vitamin B12
23 D-Biotin
C10H16N2O3S 244.316 g/mol
1 G_PER_L CHEBI:15956
biotin

Preparation

  1. 2x concentrated stock:
  2. Adjust pH to 6.8 with NaOH.
  3. Fill up to 346ml with demineralized water and filter sterilize through 0.2µm filter
  4. Aliquot 17.3ml into 50ml tubes and store at -20°C.
  5. When ready to use add:
  6. Filter sterilize each tube through 0.2µm filter.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.973523Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1600
2026-03-13T03:09:03.067085Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:01.041504Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001081
2026-03-17T06:30:08.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.713450+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "FRANCISELLA PERSICA MEDIUM" to "francisella_persica_medium" for consistent matching
2026-06-05T07:41:05.427018+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:58.785772+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:35.586278+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 5c95929992dff909dcfd29e0b764aaa0d84c63849366b3165fa90c17eb864b6c