Overview
- External ontology
- mediadive.medium:1602 (source link unavailable) — CY/3-SEAWATER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1602.pdf
Composition
flowchart LR
CultureMech_001083["cy_3_seawater_medium"]
ing0_NaCl["NaCl"]
CultureMech_001083 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001083 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_Yeast_extract["Yeast extract"]
CultureMech_001083 --> ing2_Yeast_extract
ing3_Casitone["Casitone"]
CultureMech_001083 --> ing3_Casitone
ing4_Cyanocobalamine["Cyanocobalamine"]
CultureMech_001083 --> ing4_Cyanocobalamine
CHEBI_17439["CHEBI:17439"]
ing4_Cyanocobalamine -.-> CHEBI_17439
ing5_Ferric_citrate["Ferric citrate"]
CultureMech_001083 --> ing5_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing5_Ferric_citrate -.-> CHEBI_144421
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001083 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_KCl["KCl"]
CultureMech_001083 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaHCO3["NaHCO3"]
CultureMech_001083 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_H3BO3["H3BO3"]
CultureMech_001083 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_KBr["KBr"]
CultureMech_001083 --> ing10_KBr
CHEBI_32030["CHEBI:32030"]
ing10_KBr -.-> CHEBI_32030
ing11_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_001083 --> ing11_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing11_SrCl2_x_6_H2O -.-> CHEBI_36385
ing12_Sodium_beta_glycerophosphate["Sodium beta-glycerophosphate"]
CultureMech_001083 --> ing12_Sodium_beta_glycerophosphate
CHEBI_132089["CHEBI:132089"]
ing12_Sodium_beta_glycerophosphate -.-> CHEBI_132089
ing13_EDTA["EDTA"]
CultureMech_001083 --> ing13_EDTA
CHEBI_4735["CHEBI:4735"]
ing13_EDTA -.-> CHEBI_4735
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001083 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001083 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001083 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001083 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001083 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001083 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001083 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.45 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.45, 1.0] |
| 3 | Yeast extract | 0.33 G_PER_L | ||
| 4 | Casitone | 1 G_PER_L | ||
| 5 |
Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
|
0.0005 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
|
| 6 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
0.01 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
8 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.32 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.02, 0.3] |
| 11 |
KBr
Br.K 119.002 g/mol
|
0.08 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 12 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.03 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 13 |
Sodium beta-glycerophosphate
C3H7O6P.2Na 216.037 g/mol
|
0.01 G_PER_L |
CHEBI:132089
sodium glycerol 2-phosphate
|
|
| 14 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.5 with 1M NaOH, autoclave at 121°C for 20 min. Add cyanocobalamine from a filter sterilized solution after autoclaving and cooling.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- cy_3_seawater_medium (CultureMech:001083)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.980813Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1602 |
| 2026-03-13T03:08:54.630458Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:01.063241Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001083 |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.739566+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CY/3-SEAWATER MEDIUM" to "cy_3_seawater_medium" for consistent matching |
| 2026-06-05T07:40:55.017698+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.040343+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:29.346186+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1c4298547d8581967ca2741d494081fc18d312dc0ffecffd45559e74b7ae471d |