hlh_700_medium

CultureMech:001086 bacterial COMPLEX LIQUID
Original name: HLH-700 MEDIUM

Overview

External ontology
mediadive.medium:1605 (source link unavailable) — HLH-700 MEDIUM
Applications
  • Microbial cultivation
pH
8.0
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1605.pdf

Composition

flowchart LR
    CultureMech_001086["hlh_700_medium"]
    ing0_KCl["KCl"]
    CultureMech_001086 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001086 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001086 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_K2HPO4["K2HPO4"]
    CultureMech_001086 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Na2SO4["Na2SO4"]
    CultureMech_001086 --> ing4_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing4_Na2SO4 -.-> CHEBI_32149
    ing5_Na2CO3["Na2CO3"]
    CultureMech_001086 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_TES["TES"]
    CultureMech_001086 --> ing6_TES
    CHEBI_39035["CHEBI:39035"]
    ing6_TES -.-> CHEBI_39035
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_001086 --> ing7_Yeast_extract
    ing8_Fe_NH4_citrate["Fe(NH4)citrate"]
    CultureMech_001086 --> ing8_Fe_NH4_citrate
    CHEBI_31604["CHEBI:31604"]
    ing8_Fe_NH4_citrate -.-> CHEBI_31604
    ing9_NaHCO3["NaHCO3"]
    CultureMech_001086 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001086 --> ing10_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing10_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing11_CuSO4_x_4_H2O["CuSO4 x 4 H2O"]
    CultureMech_001086 --> ing11_CuSO4_x_4_H2O
    CHEBI_23414["CHEBI:23414"]
    ing11_CuSO4_x_4_H2O -.-> CHEBI_23414
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001086 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_ZnSO4["ZnSO4"]
    CultureMech_001086 --> ing13_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing13_ZnSO4 -.-> CHEBI_35176
    ing14_H3BO3["H3BO3"]
    CultureMech_001086 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001086 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_NaNO3["NaNO3"]
    CultureMech_001086 --> ing16_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing16_NaNO3 -.-> CHEBI_63005
    ing17_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001086 --> ing17_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing17_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing18_Na2_EDTA["Na2-EDTA"]
    CultureMech_001086 --> ing18_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing18_Na2_EDTA -.-> CHEBI_64734
    ing19_Biotin["Biotin"]
    CultureMech_001086 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_001086 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001086 --> ing21_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing22_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001086 --> ing22_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing22_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing23_Riboflavin["Riboflavin"]
    CultureMech_001086 --> ing23_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing23_Riboflavin -.-> CHEBI_17015
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001086 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_001086 --> ing25_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_D_Calcium_pantothenate -.-> CHEBI_31345
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_001086 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001086 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_Lipoic_acid["Lipoic acid"]
    CultureMech_001086 --> ing28_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_Lipoic_acid -.-> CHEBI_16494

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 KCl
Cl.K 74.551 g/mol
2.609 G_PER_L CHEBI:32588
potassium chloride
2 NH4Cl
Cl.H4N 53.492 g/mol
0.267 G_PER_L CHEBI:31206
ammonium chloride
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
180.026 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 172.536, 7.49]
4 K2HPO4
HO4P.2K 174.174 g/mol
0.03 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 Na2SO4
2Na.O4S 142.043 g/mol
19.886 G_PER_L CHEBI:32149
sodium sulfate
6 Na2CO3
CO3.2Na 105.988 g/mol
0.023 G_PER_L CHEBI:29377
sodium carbonate
7 TES 2.292 G_PER_L CHEBI:39035
TES
8 Yeast extract 2.5 G_PER_L
9 Fe(NH4)citrate 0.006 G_PER_L CHEBI:31604
ferric ammonium citrate
10 NaHCO3
CHO3.Na 84.006 g/mol
0.84 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0494 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
12 CuSO4 x 4 H2O
Cu.O4S 159.609 g/mol
0.079 G_PER_L CHEBI:23414
copper(II) sulfate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.81 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 ZnSO4
O4S.Zn 161.453 g/mol
0.222 G_PER_L CHEBI:35176
zinc sulfate
15 H3BO3
H3BO3 61.833 g/mol
2.86 G_PER_L CHEBI:33118
boric acid
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.39 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 NaNO3
NO3.Na 84.994 g/mol
149.58 G_PER_L CHEBI:63005
sodium nitrate
18 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
19 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.104 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
20 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
21 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
22 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
23 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
24 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
25 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
26 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
27 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
28 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
29 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Adjust pH to 8.0
  2. Sterilize at 121°C for 15 minutes. After sterilization add 1 ml/l filter-sterilized Wolfe´s vitamin solution.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.989075Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1605
2026-01-28T19:48:14.350307Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:08:52.158663Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:01.089897Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001086
2026-03-17T06:29:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.769648+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HLH-700 MEDIUM" to "hlh_700_medium" for consistent matching
2026-06-05T07:41:08.579145+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.782457+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:37.657783+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 0a7960681a9f936c039029fb3fadfad35c32aa2a14b38672f2b3ceefa97c0ce9