methyloceanibacter_medium_nacl_danms

CultureMech:001092 bacterial DEFINED LIQUID
Original name: METHYLOCEANIBACTER MEDIUM (NaCl-dANMS)

Overview

External ontology
mediadive.medium:1610 (source link unavailable) — METHYLOCEANIBACTER MEDIUM (NaCl-dANMS)
Applications
  • Microbial cultivation
pH
7.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1610.pdf

Composition

flowchart LR
    CultureMech_001092["methyloceanibacter_medium_nacl_danms"]
    ing0_Methanol["Methanol"]
    CultureMech_001092 --> ing0_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing0_Methanol -.-> CHEBI_17790
    ing1_EDTA["EDTA"]
    CultureMech_001092 --> ing1_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing1_EDTA -.-> CHEBI_4735
    ing2_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001092 --> ing2_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing2_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing3_ZnSO4["ZnSO4"]
    CultureMech_001092 --> ing3_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing3_ZnSO4 -.-> CHEBI_35176
    ing4_MnCl2["MnCl2"]
    CultureMech_001092 --> ing4_MnCl2
    CHEBI_63041["CHEBI:63041"]
    ing4_MnCl2 -.-> CHEBI_63041
    ing5_H3BO3["H3BO3"]
    CultureMech_001092 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001092 --> ing6_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing6_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing7_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_001092 --> ing7_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing7_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001092 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001092 --> ing9_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing10_MgSO4_x_6_H2O["MgSO4 x 6 H2O"]
    CultureMech_001092 --> ing10_MgSO4_x_6_H2O
    CHEBI_32599["CHEBI:32599"]
    ing10_MgSO4_x_6_H2O -.-> CHEBI_32599
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001092 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing12_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_001092 --> ing12_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing12_Fe_III__EDTA -.-> CHEBI_30729
    ing13_KH2PO4["KH2PO4"]
    CultureMech_001092 --> ing13_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing13_KH2PO4 -.-> CHEBI_63036
    ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001092 --> ing14_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing15_NaCl["NaCl"]
    CultureMech_001092 --> ing15_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing15_NaCl -.-> CHEBI_26710
    ing16_NH4Cl["NH4Cl"]
    CultureMech_001092 --> ing16_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing16_NH4Cl -.-> CHEBI_31206
    ing17_KNO3["KNO3"]
    CultureMech_001092 --> ing17_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing17_KNO3 -.-> CHEBI_63043
    ing18_HEPES["HEPES"]
    CultureMech_001092 --> ing18_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing18_HEPES -.-> CHEBI_46756
    ing19_CeCl3_x_7_H2O["CeCl3 x 7 H2O"]
    CultureMech_001092 --> ing19_CeCl3_x_7_H2O
    CHEBI_35458["CHEBI:35458"]
    ing19_CeCl3_x_7_H2O -.-> CHEBI_35458
    ing20_LaCl3_x_7_H2O["LaCl3 x 7 H2O"]
    CultureMech_001092 --> ing20_LaCl3_x_7_H2O
    ing21_NdCl3_x_6_H2O["NdCl3 x 6 H2O"]
    CultureMech_001092 --> ing21_NdCl3_x_6_H2O
    ing22_PrCl3_x_H2O["PrCl3 x H2O"]
    CultureMech_001092 --> ing22_PrCl3_x_H2O

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 Methanol
CH4O 32.042 g/mol
7.92 G_PER_L CHEBI:17790
methanol
2 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
3 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
4 ZnSO4
O4S.Zn 161.453 g/mol
0.01 G_PER_L CHEBI:35176
zinc sulfate
5 MnCl2
Cl2Mn 125.844 g/mol
0.003 G_PER_L CHEBI:63041
manganese(II) chloride
6 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
7 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.02 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
8 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.01 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
10 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.002 G_PER_L CHEBI:75213
sodium molybdate dihydrate
11 MgSO4 x 6 H2O
Mg.O4S 120.368 g/mol
10 G_PER_L CHEBI:32599
magnesium sulfate
12 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.5 G_PER_L CHEBI:86158
calcium chloride dihydrate
13 Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
36.05 G_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
14 KH2PO4
H2O4P.K 136.084 g/mol
13.6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
15 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
2.5 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
16 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
17 NH4Cl
Cl.H4N 53.492 g/mol
5.3 G_PER_L CHEBI:31206
ammonium chloride
18 KNO3
K.NO3 101.102 g/mol
10.1 G_PER_L CHEBI:63043
potassium nitrate
19 HEPES 23.8 G_PER_L CHEBI:46756
HEPES
20 CeCl3 x 7 H2O
CeCl3 246.475 g/mol
0.04 G_PER_L CHEBI:35458
cerium trichloride
21 LaCl3 x 7 H2O 0.04 G_PER_L
22 NdCl3 x 6 H2O 0.04 G_PER_L
23 PrCl3 x H2O 0.02 G_PER_L

Preparation

  1. Dilute 20 ml of solution 1 to 700ml with distilled water.
  2. Add 100mL of solution 5, 50 mL of solution 8, 20mL of solution 6 and 7 and 10 mL of solution 3.
  3. Add 1ml trace element solution.
  4. Add 0.4 mL solution 2.
  5. Add 0.1 mL solution 4.
  6. Add 1.0 mL solution 9.
  7. Disolve and bring to 1L.
  8. Adjust pH to 7.8 with HCl or NaOH. Autoclave medium.
  9. After autoclaving add 10mL per liter of filter sterilized methanol.
  10. May be stored at 4°C in the dark
  11. Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.001672Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1610
2026-02-06T05:10:19.420022Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:01.125022Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001092
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.808923+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOCEANIBACTER MEDIUM (NaCl-dANMS)" to "methyloceanibacter_medium_nacl_danms" for consistent matching
2026-06-05T07:41:33.069612+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.515349+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.534965+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.654934+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 1a1d6e555ec0c1518465226a571bdde332888e44272fccd4e066c5dcf6c78816