Overview
- External ontology
- mediadive.medium:1624 (source link unavailable) — METHYLOPHAGA MURALIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1624.pdf
Composition
flowchart LR
CultureMech_001105["methylophaga_muralis_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001105 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_KNO3["KNO3"]
CultureMech_001105 --> ing1_KNO3
CHEBI_63043["CHEBI:63043"]
ing1_KNO3 -.-> CHEBI_63043
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001105 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NaCl["NaCl"]
CultureMech_001105 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.1236 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
KNO3
K.NO3 101.102 g/mol
|
1.1236 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.247191 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
33.7079 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- pH 5.0
- pH is adjusted to 9.0 by adding 100 ml/l 1M sterilized NaHCO3 and 10 ml/l 1M sterilized Na2CO3. 5 ml/l sterile methanol is added to the cooled medium. When preparing liquid media cool the mineral salts solution, Na2CO3 solution and NaHCO3 solution to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salts solution and autoclave. Cool the Na2CO3 solution, NaHCO3 solution and agar to 50-55°C before mixing.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methylophaga_muralis_medium (CultureMech:001105)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.033607Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1624 |
| 2026-03-13T06:19:01.220269Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001105 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.915371+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOPHAGA MURALIS MEDIUM" to "methylophaga_muralis_medium" for consistent matching |
| 2026-06-05T07:41:33.329668+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.564265+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:07.638027+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:45.049976+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d599aaf507510e99f7a302eb48e80a32f5a32b4020a9e58b7ccceb490ba6582d |