Overview
- External ontology
- mediadive.medium:1629 (source link unavailable) — MINIMAL MEDIA CONTAINING METHANOL
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1629.pdf
Composition
flowchart LR
CultureMech_001108["minimal_media_containing_methanol"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001108 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001108 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_001108 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
CultureMech_001108 --> ing3_NaH2PO4_x_2_H2O
CHEBI_37585["CHEBI:37585"]
ing3_NaH2PO4_x_2_H2O -.-> CHEBI_37585
ing4_Na2_EDTA["Na2-EDTA"]
CultureMech_001108 --> ing4_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing4_Na2_EDTA -.-> CHEBI_64734
ing5_FeSO4_x_6_H2O["FeSO4 x 6 H2O"]
CultureMech_001108 --> ing5_FeSO4_x_6_H2O
CHEBI_75832["CHEBI:75832"]
ing5_FeSO4_x_6_H2O -.-> CHEBI_75832
ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001108 --> ing6_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001108 --> ing7_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001108 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_H3BO3["H3BO3"]
CultureMech_001108 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001108 --> ing10_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing11_CuSO4_x_2_H2O["CuSO4 x 2 H2O"]
CultureMech_001108 --> ing11_CuSO4_x_2_H2O
CHEBI_23414["CHEBI:23414"]
ing11_CuSO4_x_2_H2O -.-> CHEBI_23414
ing12_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
CultureMech_001108 --> ing12_CaCl2_x_6_H2O
CHEBI_91243["CHEBI:91243"]
ing12_CaCl2_x_6_H2O -.-> CHEBI_91243
ing13_Methanol["Methanol"]
CultureMech_001108 --> ing13_Methanol
CHEBI_17790["CHEBI:17790"]
ing13_Methanol -.-> CHEBI_17790
ing14_Agar["Agar"]
CultureMech_001108 --> ing14_Agar
CHEBI_2509["CHEBI:2509"]
ing14_Agar -.-> CHEBI_2509
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.62 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
|
1.1 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 5 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.015 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 6 |
FeSO4 x 6 H2O
Fe.O4S 151.908 g/mol
|
0.003 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 7 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0045 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 8 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.003 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.00064 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.001 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0004 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 12 |
CuSO4 x 2 H2O
Cu.O4S 159.609 g/mol
|
0.0003 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
|
| 13 |
CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
|
0.003 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
|
| 14 |
Methanol
CH4O 32.042 g/mol
|
3.96 G_PER_L |
CHEBI:17790
methanol
|
|
| 15 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Prepare the medium without methanol. Adjust pH to 7.1.
- Autoclave and after cooling to 50°C add the methanol from a filter-sterilized stock solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- minimal_media_containing_methanol (CultureMech:001108)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.041736Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1629 |
| 2026-03-13T06:19:01.234655Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001108 |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.931688+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MINIMAL MEDIA CONTAINING METHANOL" to "minimal_media_containing_methanol" for consistent matching |
| 2026-06-05T07:41:34.650225+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.062987+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.322180+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 18947c2197e2cdbb83d290e3a1c1fe37678a0b6361e9614ac2f7591eb3846fb4 |