Overview
- External ontology
- mediadive.medium:162 (source link unavailable) — HYPHOMICROBIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium162.pdf
Composition
flowchart LR
CultureMech_001109["hyphomicrobium_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001109 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4["Na2HPO4"]
CultureMech_001109 --> ing1_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4 -.-> CHEBI_34683
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_001109 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001109 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001109 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001109 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001109 --> ing6_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing6_MnSO4_x_H2O -.-> CHEBI_86364
ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001109 --> ing7_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing8_Methylamine_hydrochloride["Methylamine hydrochloride"]
CultureMech_001109 --> ing8_Methylamine_hydrochloride
CHEBI_59337["CHEBI:59337"]
ing8_Methylamine_hydrochloride -.-> CHEBI_59337
ing9_Yeast_extract["Yeast extract"]
CultureMech_001109 --> ing9_Yeast_extract
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.36 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
2.13 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.006 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.003 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.001 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 8 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0015 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 9 |
Methylamine hydrochloride
CH6N.Cl 67.519 g/mol
|
3.38 G_PER_L |
CHEBI:59337
methylamine hydrochloride
|
|
| 10 | Yeast extract | 0.1 G_PER_L |
Preparation
- Adjust pH to 7.4 with NaOH. Autoclave 20 min at 121°C; final pH: 7.2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- DSMZ_162_HYPHOMICROBIUM_MEDIUM (CultureMech:001109)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.377814Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 162 |
| 2026-03-13T06:19:01.240627Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001109 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.938642+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HYPHOMICROBIUM MEDIUM" to "hyphomicrobium_medium" for consistent matching |
| 2026-06-05T07:40:16.096212+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.618965+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:34.543367+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c8032f6a503d33a048c2fa3f57e6708dd639bda6f5135d97cdab99179344dd48 |