modified_nitrosospaera_medium

Overview

External ontology

mediadive.medium:1630c (source link unavailable) — modified Nitrosospaera medium

Applications

• Microbial cultivation

pH

7.5

High-metal flag

True

Notes

Source: DSMZ

Composition

flowchart LR
    CultureMech_001113["modified_nitrosospaera_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001113 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001113 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001113 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_001113 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_KCl["KCl"]
    CultureMech_001113 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Na2SO4["Na2SO4"]
    CultureMech_001113 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_Double_distilled_water["Double distilled water"]
    CultureMech_001113 --> ing6_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Double_distilled_water -.-> CHEBI_15377
    ing7_HCl["HCl"]
    CultureMech_001113 --> ing7_HCl
    CHEBI_17883["CHEBI:17883"]
    ing7_HCl -.-> CHEBI_17883
    ing8_H3BO3["H3BO3"]
    CultureMech_001113 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001113 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001113 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001113 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001113 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001113 --> ing13_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001113 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_NaOH["NaOH"]
    CultureMech_001113 --> ing15_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing15_NaOH -.-> CHEBI_32145
    ing16_HEPES["HEPES"]
    CultureMech_001113 --> ing16_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing16_HEPES -.-> CHEBI_46756
    ing17_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_001113 --> ing17_FeNa_EDTA
    ing18_NaHCO3["NaHCO3"]
    CultureMech_001113 --> ing18_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing18_NaHCO3 -.-> CHEBI_32139
    ing19_NH4Cl["NH4Cl"]
    CultureMech_001113 --> ing19_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing19_NH4Cl -.-> CHEBI_31206
    ing20_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_001113 --> ing20_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing20_Sodium_pyruvate -.-> CHEBI_50144

Ingredients (21)

Preparation

1. Incubate all used glass ware and magnetic stirring rods in 0.1 M HCl and rinse 3x with double distilled water to remove residual detergents (always check visual for soap bubbles).
2. Autoclave the Basic-FWM and cool to room temperature. This medium should be free of any precipitations!
3. Aseptically, add per L of BASIC-FWM the following solutions from sterile stocks (do not store complemented FWM): - Trace element solution (Nitrososphaera) - FeNaEDTA solution - NaHCO3 solution - HEPES buffer solution - NH4Cl solution - Sodiumpyruvate solution
4. Adjust pH to 7.5.
5. This solution contains no iron! Sterilise by autoclaving or filtration (0.2 µm) and store in the dark at 4 °C.
6. Fist dissolve NaOH in ca 600 ml water, then add HEPES, adjust pH to 7.5 and fill up to 1000 ml volume.
7. Sterilise by filtration (0.2 µm) and store in the dark at 4 °C.
8. Dissolve 4.2 g of NaHCO3 in 50 mL Double distilled water (use a small beaker with stirring rod to dissolve) and sterilize by filtration (0.2 µm) in the laminar flow hood. Store in a sterile 50 mL falcon tube at 4 °C. Avoid high headspace to liquid ratios to ensure stable concentration.
9. Dissolve 2.67 g ammonium chloride in 50 mL Double distilled water.
10. Filtrate (0.2 µm).
11. Dissolve 5.5 g in 50 mL and filter it through 0.2 µm sterile filter in a sterile falcon tube. Store at 4 °C

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• modified_nitrosospaera_medium (CultureMech:001113)