serpentinomonas_minimal_medium

CultureMech:001118 bacterial DEFINED SOLID_AGAR
Original name: SERPENTINOMONAS MINIMAL MEDIUM

Overview

External ontology
mediadive.medium:1634 (source link unavailable) — SERPENTINOMONAS MINIMAL MEDIUM
Applications
  • Microbial cultivation
pH
11.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1634.pdf

Composition

flowchart LR
    CultureMech_001118["serpentinomonas_minimal_medium"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_001118 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001118 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001118 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_CAPS_buffer["CAPS buffer"]
    CultureMech_001118 --> ing3_CAPS_buffer
    CHEBI_191088["CHEBI:191088"]
    ing3_CAPS_buffer -.-> CHEBI_191088
    ing4_Agar["Agar"]
    CultureMech_001118 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_Double_distilled_water["Double distilled water"]
    CultureMech_001118 --> ing5_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Double_distilled_water -.-> CHEBI_15377
    ing6_K2HPO4["K2HPO4"]
    CultureMech_001118 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_CaCl2["CaCl2"]
    CultureMech_001118 --> ing7_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing7_CaCl2 -.-> CHEBI_3312
    ing8_Na_acetate["Na acetate"]
    CultureMech_001118 --> ing8_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Na_acetate -.-> CHEBI_32954
    ing9_Folic_acid["Folic acid"]
    CultureMech_001118 --> ing9_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing9_Folic_acid -.-> CHEBI_27470
    ing10_Biotin["Biotin"]
    CultureMech_001118 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_Riboflavin["Riboflavin"]
    CultureMech_001118 --> ing11_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing11_Riboflavin -.-> CHEBI_17015
    ing12_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001118 --> ing12_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing12_Nicotinic_acid -.-> CHEBI_15940
    ing13_Thiamine["Thiamine"]
    CultureMech_001118 --> ing13_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing13_Thiamine -.-> CHEBI_18385
    ing14_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001118 --> ing14_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing14_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001118 --> ing15_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing16_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001118 --> ing16_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_pantothenate -.-> CHEBI_31345
    ing17_Vitamin_B12["Vitamin B12"]
    CultureMech_001118 --> ing17_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing17_Vitamin_B12 -.-> CHEBI_176843
    ing18_Thioctic_acid["Thioctic acid"]
    CultureMech_001118 --> ing18_Thioctic_acid
    CHEBI_30314["CHEBI:30314"]
    ing18_Thioctic_acid -.-> CHEBI_30314
    ing19_Monopotassium_Phosphate["Monopotassium Phosphate"]
    CultureMech_001118 --> ing19_Monopotassium_Phosphate
    CHEBI_63036["CHEBI:63036"]
    ing19_Monopotassium_Phosphate -.-> CHEBI_63036
    ing20_Na_EDTA_x_2_H2O["Na-EDTA x 2 H2O"]
    CultureMech_001118 --> ing20_Na_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing20_Na_EDTA_x_2_H2O -.-> CHEBI_64758
    ing21_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001118 --> ing21_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing21_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing22_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001118 --> ing22_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing22_MnSO4_x_H2O -.-> CHEBI_86364
    ing23_NaCl["NaCl"]
    CultureMech_001118 --> ing23_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing23_NaCl -.-> CHEBI_26710
    ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001118 --> ing24_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing25_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001118 --> ing25_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing25_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing26_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001118 --> ing26_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing26_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing27_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001118 --> ing27_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing27_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing28_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_001118 --> ing28_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing28_AlK_SO4_2 -.-> CHEBI_86463
    ing29_H3BO3["H3BO3"]
    CultureMech_001118 --> ing29_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing29_H3BO3 -.-> CHEBI_33118
    CultureMech_001118 --> more["...4 more"]

Ingredients (34)

# Ingredient Concentration Ontology Notes
1 Na2SO4
2Na.O4S 142.043 g/mol
0.0284 G_PER_L CHEBI:32149
sodium sulfate
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.0404 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.0802 G_PER_L CHEBI:31206
ammonium chloride
4 CAPS buffer
C9H19NO3S 221.322 g/mol
3.32 G_PER_L CHEBI:191088
Cyclohexylaminopropanesulfonic acid
5 Agar 15 G_PER_L CHEBI:2509
agar
 Optional ingredient (if required)
6 Double distilled water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water
7 K2HPO4
HO4P.2K 174.174 g/mol
0.0522528 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 CaCl2
Ca.2Cl 110.984 g/mol
0.835075 G_PER_L CHEBI:3312
calcium dichloride
 [Merged 2 duplicates: 0.735075, 0.1]
9 Na acetate
C2H3O2.Na 82.034 g/mol
0.328135 G_PER_L CHEBI:32954
sodium acetate
10 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
11 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
12 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
13 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
14 Thiamine
C12H17N4OS 265.362 g/mol
0.005 G_PER_L CHEBI:18385
thiamine(1+)
15 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
16 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
17 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
18 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
19 Thioctic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:30314
(R)-lipoic acid
20 Monopotassium Phosphate
H2O4P.K 136.084 g/mol
0.9 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
21 Na-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
0.5 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
22 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
23 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
24 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
25 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
26 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.1 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
27 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
28 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
29 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
30 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
31 Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
32 Na2SeO3
2Na.O3Se 172.937 g/mol
0.001 G_PER_L CHEBI:48843
disodium selenite
33 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.01 G_PER_L CHEBI:63939
sodium tungstate dihydrate
34 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride

Preparation

  1. Adjust pH to 11.0 with NaOH.
  2. Fill into screw-cap tubes, leaving 60% headspace.
  3. Sparge medium with 100% N2 gas mixture to make it anoxic.
  4. Autoclave at 121 °C for 20 min.
  5. After autoclaving, cool down and add from sterile stock solutions while stirring:
  6. Add 30% air.
  7. Note: Some precipitate will form.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.078922Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1634
2026-01-28T19:48:22.157387Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:06.517166Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:01.352928Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001118
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.061093+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SERPENTINOMONAS MINIMAL MEDIUM" to "serpentinomonas_minimal_medium" for consistent matching
2026-06-05T07:41:45.748025+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.456354+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:55.706211+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 9ac5b2f477f6aefce9559795dabb6706494dd281a288ff18ea0c251d677bb635