methanomethylophilus_medium

Overview

External ontology

mediadive.medium:1637 (source link unavailable) — METHANOMETHYLOPHILUS MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.6, 'max': 7.8}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1637.pdf

Composition

flowchart LR
    CultureMech_001121["methanomethylophilus_medium"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001121 --> ing0_Trypticase_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001121 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_001121 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001121 --> ing3_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing3_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing4_NaHCO3["NaHCO3"]
    CultureMech_001121 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001121 --> ing5_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Sodium_resazurin -.-> CHEBI_8806
    ing6_Methanol["Methanol"]
    CultureMech_001121 --> ing6_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing6_Methanol -.-> CHEBI_17790
    ing7_DL_Dithiothreitol["DL-Dithiothreitol"]
    CultureMech_001121 --> ing7_DL_Dithiothreitol
    CHEBI_18320["CHEBI:18320"]
    ing7_DL_Dithiothreitol -.-> CHEBI_18320
    ing8_Haemin["Haemin"]
    CultureMech_001121 --> ing8_Haemin
    CHEBI_50385["CHEBI:50385"]
    ing8_Haemin -.-> CHEBI_50385
    ing9_NaOH["NaOH"]
    CultureMech_001121 --> ing9_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing9_NaOH -.-> CHEBI_32145
    ing10_Vitamin_K1["Vitamin K1"]
    CultureMech_001121 --> ing10_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing10_Vitamin_K1 -.-> CHEBI_18067
    ing11_Ethanol["Ethanol"]
    CultureMech_001121 --> ing11_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing11_Ethanol -.-> CHEBI_16236

Ingredients (12)

Preparation

1. Dissolve ingredients except for methanol and dithiothreitol. Adjust pH to 7.5 with 1 N NaOH and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After sterilization add methanol from a sterile anoxic stock solution (15% v/v) autoclaved under 100% N2 gas atmosphere and dithiothreitol from an anoxic stock solution sterilized by filtration. Before use check the pH of the complete medium and adjust it to 7.6 - 7.8, if necessary.
2. After inoculation, add sterile 80% H2 and 20% CO2 gas mixture to 0.5 bar overpressure. After growth becomes visible overpressure of 80% H2 and 20% CO2 gas mixture can be increased to 1 bar.
3. Note: Use 10% (v/v) as inoculum.
4. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.
5. Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• methanomethylophilus_medium (CultureMech:001121)