azoarcus_taiwanensis_medium

CultureMech:001123 bacterial DEFINED LIQUID
Original name: AZOARCUS TAIWANENSIS MEDIUM

Overview

External ontology
mediadive.medium:1639 (source link unavailable) — AZOARCUS TAIWANENSIS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 8.5, 'max': 9.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1639.pdf

Composition

flowchart LR
    CultureMech_001123["azoarcus_taiwanensis_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001123 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KNO3["KNO3"]
    CultureMech_001123 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001123 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_K2HPO4["K2HPO4"]
    CultureMech_001123 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Na_acetate["Na-acetate"]
    CultureMech_001123 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5_NaHCO3["NaHCO3"]
    CultureMech_001123 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_Na_pyruvate["Na-pyruvate"]
    CultureMech_001123 --> ing6_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Na_pyruvate -.-> CHEBI_50144
    ing7_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_001123 --> ing7_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing7_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001123 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_ZnCl2["ZnCl2"]
    CultureMech_001123 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001123 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_001123 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001123 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001123 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001123 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001123 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
9.99001 G_PER_L CHEBI:26710
sodium chloride
2 KNO3
K.NO3 101.102 g/mol
0.759241 G_PER_L CHEBI:63043
potassium nitrate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.0999001 G_PER_L CHEBI:31206
ammonium chloride
4 K2HPO4
HO4P.2K 174.174 g/mol
0.24975 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 Na-acetate
C2H3O2.Na 82.034 g/mol
0.43956 G_PER_L CHEBI:32954
sodium acetate
6 NaHCO3
CHO3.Na 84.006 g/mol
1.4985 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 Na-pyruvate
C3H3O3.Na 110.044 g/mol
1.998 G_PER_L CHEBI:50144
sodium pyruvate
8 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
5.2 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
9 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
10 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Dissolve ingredients (except bicarbonate and pyruvate) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then, add and dissolve bicarbonate, adjust pH to 8.5 - 9.0, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials, and autoclave. After sterilization, add pyruvate from a sterile, anoxic stock solution that has been prepared under a 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 8.5 - 9.0, if necessary.
  2. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.097141Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1639
2026-03-13T06:19:01.402953Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001123
2026-03-17T06:30:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.115794+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AZOARCUS TAIWANENSIS MEDIUM" to "azoarcus_taiwanensis_medium" for consistent matching
2026-05-20T06:01:09.357225+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '8.5-9' -> {'min': 8.5, 'max': 9.0}
2026-06-05T07:40:50.135604+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.560456+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:53.663986+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:24.499993+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 85cf3d6f4b3a952fdda9ac164e2277f39655d2a49f2eadf77d7398d66f97c1bc