Overview
- External ontology
- mediadive.medium:1647 (source link unavailable) — ACETOBACTEROIDES MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1647.pdf
Composition
flowchart LR
CultureMech_001130["acetobacteroides_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001130 --> ing0_Yeast_extract
ing1_Glucose["Glucose"]
CultureMech_001130 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_KH2PO4["KH2PO4"]
CultureMech_001130 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001130 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001130 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_NH4Cl["NH4Cl"]
CultureMech_001130 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001130 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_NaHCO3["NaHCO3"]
CultureMech_001130 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001130 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
ing9_Resazurin["Resazurin"]
CultureMech_001130 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_Na2SeO3["Na2SeO3"]
CultureMech_001130 --> ing10_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing10_Na2SeO3 -.-> CHEBI_48843
ing11_Na2WO4["Na2WO4"]
CultureMech_001130 --> ing11_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing11_Na2WO4 -.-> CHEBI_63940
ing12_Biotin["Biotin"]
CultureMech_001130 --> ing12_Biotin
CHEBI_15956["CHEBI:15956"]
ing12_Biotin -.-> CHEBI_15956
ing13_Folic_acid["Folic acid"]
CultureMech_001130 --> ing13_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing13_Folic_acid -.-> CHEBI_27470
ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001130 --> ing14_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing15_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001130 --> ing15_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing15_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing16_Riboflavin["Riboflavin"]
CultureMech_001130 --> ing16_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing16_Riboflavin -.-> CHEBI_17015
ing17_Nicotinic_acid["Nicotinic acid"]
CultureMech_001130 --> ing17_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing17_Nicotinic_acid -.-> CHEBI_15940
ing18_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_001130 --> ing18_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing18_D_Calcium_pantothenate -.-> CHEBI_31345
ing19_Vitamin_B12["Vitamin B12"]
CultureMech_001130 --> ing19_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing19_Vitamin_B12 -.-> CHEBI_176843
ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001130 --> ing20_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
ing21_Lipoic_acid["Lipoic acid"]
CultureMech_001130 --> ing21_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing21_Lipoic_acid -.-> CHEBI_16494
ing22_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001130 --> ing22_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing22_Nitrilotriacetic_acid -.-> CHEBI_44557
ing23_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001130 --> ing23_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing23_FeCl2_x_4_H2O -.-> CHEBI_86249
ing24_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001130 --> ing24_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing24_MnCl2_x_4_H2O -.-> CHEBI_86368
ing25_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001130 --> ing25_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing25_CoCl2_x_6_H2O -.-> CHEBI_53503
ing26_ZnCl2["ZnCl2"]
CultureMech_001130 --> ing26_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing26_ZnCl2 -.-> CHEBI_49976
ing27_CuCl2["CuCl2"]
CultureMech_001130 --> ing27_CuCl2
CHEBI_49553["CHEBI:49553"]
ing27_CuCl2 -.-> CHEBI_49553
ing28_H3BO3["H3BO3"]
CultureMech_001130 --> ing28_H3BO3
CHEBI_33118["CHEBI:33118"]
ing28_H3BO3 -.-> CHEBI_33118
ing29_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001130 --> ing29_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing29_Na2MoO4_x_2_H2O -.-> CHEBI_75213
CultureMech_001130 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.2 G_PER_L | ||
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
0.18 G_PER_L |
CHEBI:17234
glucose
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.14 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.15, 0.1] |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.824796 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.3 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.004 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 12 |
Na2WO4
2Na.O4W 293.816 g/mol
|
0.004 G_PER_L |
CHEBI:63940
sodium tungstate
|
|
| 13 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 14 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 15 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 16 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 17 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 18 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 19 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 20 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 21 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 22 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 23 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 24 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 25 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 26 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.03 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 27 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 28 |
CuCl2
Cl2Cu 134.452 g/mol
|
0.02 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 29 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 30 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 31 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.1 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 32 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 33 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.03 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 34 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.04 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- The vitamin solution, trace element solution, Se/W solution, cysteine, glucose, Na2S and NaHCO3 are added after the medium has been autoclaved.
- Note: for preparation see Medium 141
- First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- acetobacteroides_medium (CultureMech:001130)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.112620Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1647 |
| 2026-01-28T19:48:23.887670Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:09.369218Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:01.457940Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001130 |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.177187+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACETOBACTEROIDES MEDIUM" to "acetobacteroides_medium" for consistent matching |
| 2026-06-05T07:40:46.875884+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.430797+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:21.628887+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b1a42f76e65b9365eb88eee37fc3c241310fdd6d04e86a88e79dbe62fd072f9e |