Overview
- External ontology
- mediadive.medium:1651 (source link unavailable) — PLANCTOMYCES MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1651.pdf
Composition
flowchart LR
CultureMech_001133["planctomyces_medium"]
ing0_Peptone["Peptone"]
CultureMech_001133 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001133 --> ing1_Yeast_extract
ing2_HEPES["HEPES"]
CultureMech_001133 --> ing2_HEPES
CHEBI_46756["CHEBI:46756"]
ing2_HEPES -.-> CHEBI_46756
ing3_Agar["Agar"]
CultureMech_001133 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Double_distilled_water["Double distilled water"]
CultureMech_001133 --> ing4_Double_distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Double_distilled_water -.-> CHEBI_15377
ing5_N_Acetylglucosamine["N-Acetylglucosamine"]
CultureMech_001133 --> ing5_N_Acetylglucosamine
CHEBI_59640["CHEBI:59640"]
ing5_N_Acetylglucosamine -.-> CHEBI_59640
ing6_NaCl["NaCl"]
CultureMech_001133 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Na2SO4["Na2SO4"]
CultureMech_001133 --> ing7_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing7_Na2SO4 -.-> CHEBI_32149
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001133 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_CaCl2["CaCl2"]
CultureMech_001133 --> ing9_CaCl2
CHEBI_3312["CHEBI:3312"]
ing9_CaCl2 -.-> CHEBI_3312
ing10_NaHCO3["NaHCO3"]
CultureMech_001133 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_KCl["KCl"]
CultureMech_001133 --> ing11_KCl
CHEBI_32588["CHEBI:32588"]
ing11_KCl -.-> CHEBI_32588
ing12_KBr["KBr"]
CultureMech_001133 --> ing12_KBr
CHEBI_32030["CHEBI:32030"]
ing12_KBr -.-> CHEBI_32030
ing13_H3BO3["H3BO3"]
CultureMech_001133 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_SrCl2["SrCl2"]
CultureMech_001133 --> ing14_SrCl2
CHEBI_36383["CHEBI:36383"]
ing14_SrCl2 -.-> CHEBI_36383
ing15_NaF["NaF"]
CultureMech_001133 --> ing15_NaF
CHEBI_28741["CHEBI:28741"]
ing15_NaF -.-> CHEBI_28741
ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001133 --> ing16_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
ing17_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001133 --> ing17_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing17_MgSO4_x_7_H2O -.-> CHEBI_31795
ing18_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001133 --> ing18_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing18_CaCl2_x_2_H2O -.-> CHEBI_86158
ing19_Na2MoO7O4_x_2_H2O["Na2MoO7O4 x 2 H2O"]
CultureMech_001133 --> ing19_Na2MoO7O4_x_2_H2O
ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001133 --> ing20_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
ing21_Na_EDTA["Na-EDTA"]
CultureMech_001133 --> ing21_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing21_Na_EDTA -.-> CHEBI_64734
ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001133 --> ing22_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing23_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001133 --> ing23_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing23_MnSO4_x_H2O -.-> CHEBI_86364
ing24_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001133 --> ing24_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing24_CuSO4_x_5_H2O -.-> CHEBI_31440
ing25_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_001133 --> ing25_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing25_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
ing26_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_001133 --> ing26_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing26_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing27_Na_Nitrilotriacetat["Na-Nitrilotriacetat"]
CultureMech_001133 --> ing27_Na_Nitrilotriacetat
ing28_Fe_SO4_x_7_H2O["Fe SO4 x 7 H2O"]
CultureMech_001133 --> ing28_Fe_SO4_x_7_H2O
ing28_Fe_SO4_x_7_H2O -.-> CHEBI_75836
ing29_ZnCl2["ZnCl2"]
CultureMech_001133 --> ing29_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing29_ZnCl2 -.-> CHEBI_49976
CultureMech_001133 --> more["...14 more"]
Ingredients (44)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.25 G_PER_L | ||
| 2 | Yeast extract | 0.25 G_PER_L | ||
| 3 | HEPES | 2.38 G_PER_L |
CHEBI:46756
HEPES
|
|
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 5 |
Double distilled water
H2O 18.015 g/mol
|
3730.0 G_PER_L |
CHEBI:15377
water
|
[Merged 4 duplicates: 730.0, 1000.0, 1000.0, 1000.0] |
| 6 | N-Acetylglucosamine | 0.8 G_PER_L |
CHEBI:59640
N-acetylglucosamine
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
46.94 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
Na2SO4
2Na.O4S 142.043 g/mol
|
7.84 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
21.28 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
CaCl2
Ca.2Cl 110.984 g/mol
|
2.86 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.38 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
KCl
Cl.K 74.551 g/mol
|
1.38 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 13 |
KBr
Br.K 119.002 g/mol
|
0.192 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.062 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.052, 0.01] |
| 15 |
SrCl2
2Cl.Sr 158.526 g/mol
|
0.08 G_PER_L |
CHEBI:36383
strontium dichloride
|
|
| 16 |
NaF
F.Na 41.988 g/mol
|
0.006 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 17 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 18 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 19 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.34 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 20 | Na2MoO7O4 x 2 H2O | 0.01267 G_PER_L | ||
| 21 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.599 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.099, 0.5] |
| 22 |
Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 23 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.095 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 24 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.654 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
[Merged 2 duplicates: 0.154, 0.5] |
| 25 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.0492 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
[Merged 2 duplicates: 0.0392, 0.01] |
| 26 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.12480000000000001 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
[Merged 2 duplicates: 0.0248, 0.1] |
| 27 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0177 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 28 | Na-Nitrilotriacetat | 1.5 G_PER_L | ||
| 29 |
Fe SO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 30 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 31 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.05 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 32 |
H2SeO3
H2O3Se 128.973 g/mol
|
0.05 G_PER_L |
CHEBI:26642
selenous acid
|
|
| 33 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 34 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 35 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.01 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 36 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.004 G_PER_L |
CHEBI:15956
biotin
|
|
| 37 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.004 G_PER_L |
CHEBI:27470
folic acid
|
|
| 38 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.02 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 39 |
Riboflavine
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 40 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.01 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 41 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.01 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 42 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.01 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 43 | Vitamin B12 | 0.0002 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 44 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Preparation
- Sterilize 20 min./121°C. After cooling to 60°C add:
- Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH).
- Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- planctomyces_medium (CultureMech:001133)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.125448Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1651 |
| 2026-01-28T19:48:25.991884Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:13.199474Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 8 duplicate ingredient(s) |
| 2026-03-13T06:19:01.501649Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001133 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.225855+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PLANCTOMYCES MEDIUM" to "planctomyces_medium" for consistent matching |
| 2026-06-05T07:41:42.124185+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 39 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.272537+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.136038+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9f041a1b31b73920cecb49e1b91755ffdeec5c45c3e62412bc4d7107d8c1ccae |