Overview
- External ontology
- mediadive.medium:1653 (source link unavailable) — METHYLOMONAS MEDIUM (ELBE)
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1653.pdf
Composition
flowchart LR
CultureMech_001135["methylomonas_medium_elbe"]
ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001135 --> ing0_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
ing1_KCl["KCl"]
CultureMech_001135 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001135 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_HEPES_buffer["HEPES buffer"]
CultureMech_001135 --> ing3_HEPES_buffer
CHEBI_46756["CHEBI:46756"]
ing3_HEPES_buffer -.-> CHEBI_46756
ing4_Vitamin_B12["Vitamin B12"]
CultureMech_001135 --> ing4_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing4_Vitamin_B12 -.-> CHEBI_176843
ing5_Agar["Agar"]
CultureMech_001135 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001135 --> ing6_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing6_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing7_KH2PO4["KH2PO4"]
CultureMech_001135 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_HCl["HCl"]
CultureMech_001135 --> ing8_HCl
CHEBI_17883["CHEBI:17883"]
ing8_HCl -.-> CHEBI_17883
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001135 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_ZnCl2["ZnCl2"]
CultureMech_001135 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001135 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_001135 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001135 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001135 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001135 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001135 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_Biotin["Biotin"]
CultureMech_001135 --> ing17_Biotin
CHEBI_15956["CHEBI:15956"]
ing17_Biotin -.-> CHEBI_15956
ing18_Para_aminobenzoic_acid["Para-aminobenzoic acid"]
CultureMech_001135 --> ing18_Para_aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing18_Para_aminobenzoic_acid -.-> CHEBI_30753
ing19_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001135 --> ing19_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing19_Calcium_pantothenate -.-> CHEBI_31345
ing20_Pyridoxamine_Dihydrochloride["Pyridoxamine Dihydrochloride"]
CultureMech_001135 --> ing20_Pyridoxamine_Dihydrochloride
CHEBI_131532["CHEBI:131532"]
ing20_Pyridoxamine_Dihydrochloride -.-> CHEBI_131532
ing21_Nicotinic_acid["Nicotinic acid"]
CultureMech_001135 --> ing21_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing21_Nicotinic_acid -.-> CHEBI_15940
ing22_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001135 --> ing22_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing22_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
9.27 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
10 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.22 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 | HEPES buffer | 2.383 G_PER_L |
CHEBI:46756
HEPES
|
|
| 5 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 7 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.9 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Para-aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 20 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.01 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 21 |
Pyridoxamine Dihydrochloride
C8H12N2O2.2HCl 241.118 g/mol
|
0.25 G_PER_L |
CHEBI:131532
pyridoxamine dihydrochloride
|
|
| 22 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.1 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 23 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.02 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methylomonas_medium_elbe (CultureMech:001135)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:15.134309Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1653 |
| 2026-03-13T06:19:01.521189Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001135 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.248656+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOMONAS MEDIUM (ELBE)" to "methylomonas_medium_elbe" for consistent matching |
| 2026-06-05T07:41:33.203697+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.587638+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:44.898227+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9e1177243dfd46b1bfcc7c08d91c2d41aa4b3f4c0f49c2f89e957c456d1dcd4d |