Overview
- External ontology
- mediadive.medium:1659 (source link unavailable) — RHODOBACTER MARINUS MEDIUM (NKPB)
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1659.pdf
Composition
flowchart LR
CultureMech_001141["rhodobacter_marinus_medium_nkpb"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001141 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_001141 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001141 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001141 --> ing3_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
ing4_Na2_EDTA["Na2-EDTA"]
CultureMech_001141 --> ing4_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing4_Na2_EDTA -.-> CHEBI_64734
ing5_NH4Cl["NH4Cl"]
CultureMech_001141 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_NaCl["NaCl"]
CultureMech_001141 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001141 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_DL_Malate["DL-Malate"]
CultureMech_001141 --> ing8_DL_Malate
CHEBI_15595["CHEBI:15595"]
ing8_DL_Malate -.-> CHEBI_15595
ing9_Yeast_extract["Yeast extract"]
CultureMech_001141 --> ing9_Yeast_extract
ing10_Peptone["Peptone"]
CultureMech_001141 --> ing10_Peptone
ing11_Biotin["Biotin"]
CultureMech_001141 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_H3BO3["H3BO3"]
CultureMech_001141 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001141 --> ing13_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing13_MnSO4_x_H2O -.-> CHEBI_86364
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001141 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001141 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_Cu_NO3_2_x_3_H2O["Cu(NO3)2 x 3 H2O"]
CultureMech_001141 --> ing16_Cu_NO3_2_x_3_H2O
CHEBI_78036["CHEBI:78036"]
ing16_Cu_NO3_2_x_3_H2O -.-> CHEBI_78036
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.012 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 5 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.02 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.075 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
DL-Malate
C4H4O5 132.071 g/mol
|
6 G_PER_L |
CHEBI:15595
malate(2-)
|
|
| 10 | Yeast extract | 0.5 G_PER_L | ||
| 11 | Peptone | 0.5 G_PER_L | ||
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1.5e-05 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
2.8 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
2.1 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.75 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.24 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
Cu(NO3)2 x 3 H2O
Cu.2NO3 187.554 g/mol
|
0.04 G_PER_L |
CHEBI:78036
copper(II) nitrate
|
Preparation
- Adjust pH to 7.6 with NaOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- rhodobacter_marinus_medium_nkpb (CultureMech:001141)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.150229Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1659 |
| 2026-03-13T06:19:01.561814Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001141 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.295001+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RHODOBACTER MARINUS MEDIUM (NKPB)" to "rhodobacter_marinus_medium_nkpb" for consistent matching |
| 2026-06-05T07:41:44.278936+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.937230+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.653381+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 50511e25f90a1fd718706183db2ded0b0164cb6ad1d049b86668de34b36db93b |