Overview
- External ontology
- mediadive.medium:1661 (source link unavailable) — ALKALIPHILUS NAMSARAEVII MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1661.pdf
Composition
flowchart LR
CultureMech_001144["alkaliphilus_namsaraevii_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001144 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NaCl["NaCl"]
CultureMech_001144 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Yeast_extract["Yeast extract"]
CultureMech_001144 --> ing2_Yeast_extract
ing3_NaHCO3["NaHCO3"]
CultureMech_001144 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Na2CO3["Na2CO3"]
CultureMech_001144 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_Fe_III__citrate["Fe(III) citrate"]
CultureMech_001144 --> ing5_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing5_Fe_III__citrate -.-> CHEBI_144421
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001144 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_HCl["HCl"]
CultureMech_001144 --> ing7_HCl
CHEBI_17883["CHEBI:17883"]
ing7_HCl -.-> CHEBI_17883
ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001144 --> ing8_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
ing9_ZnCl2["ZnCl2"]
CultureMech_001144 --> ing9_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing9_ZnCl2 -.-> CHEBI_49976
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001144 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_H3BO3["H3BO3"]
CultureMech_001144 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001144 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001144 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001144 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001144 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_NaOH["NaOH"]
CultureMech_001144 --> ing16_NaOH
CHEBI_32145["CHEBI:32145"]
ing16_NaOH -.-> CHEBI_32145
ing17_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001144 --> ing17_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing17_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001144 --> ing18_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing19_Biotin["Biotin"]
CultureMech_001144 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_Folic_acid["Folic acid"]
CultureMech_001144 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001144 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Thiamine_HCl["Thiamine HCl"]
CultureMech_001144 --> ing22_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing22_Thiamine_HCl -.-> CHEBI_49105
ing23_Riboflavin["Riboflavin"]
CultureMech_001144 --> ing23_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing23_Riboflavin -.-> CHEBI_17015
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_001144 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001144 --> ing25_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing25_Calcium_D_____pantothenate -.-> CHEBI_31345
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_001144 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001144 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001144 --> ing28__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.498504 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
24.9252 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 | Yeast extract | 2.49252 G_PER_L | ||
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.84447 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.19641 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 6 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
7.27817 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.199402 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 9 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 10 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 18 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 19 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 23 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 24 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 26 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 27 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 29 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except bicarbonate, carbonate, trace elements, ferric citrate, magnesium chloride and vitamins) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and carbonate, then adjust pH to 9.5 and dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add ferric citrate (10% w/v stock solution; dissolve by boiling and adjust pH to 7.5-8.0), trace elements, selenite-tungstate solution, magnesium chloride and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas. Sterilize vitamins by filtration. Adjust pH of the complete medium to 9.5, if necessary.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- alkaliphilus_namsaraevii_medium (CultureMech:001144)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.156129Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1661 |
| 2026-02-06T05:10:36.609318Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:01.598854Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001144 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.336469+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALIPHILUS NAMSARAEVII MEDIUM" to "alkaliphilus_namsaraevii_medium" for consistent matching |
| 2026-06-05T07:40:48.177029+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.902032+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.648314+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0daabb0eca5fb73cc42fe6450a6da5f419ea480eb91ec126e132de53165c9870 |