Overview
- External ontology
- mediadive.medium:1678 (source link unavailable) — Cyanobacteria Medium BA+50+B+N/2
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1678.pdf
Composition
flowchart LR
CultureMech_001160["cyanobacteria_medium_ba_50_b_n_2"]
ing0_NaCl["NaCl"]
CultureMech_001160 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001160 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KCl["KCl"]
CultureMech_001160 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Mg_SO4__x_7_H2O["Mg(SO4) x 7 H2O"]
CultureMech_001160 --> ing3_Mg_SO4__x_7_H2O
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001160 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Na3_citrate_x_2_H2O["Na3-citrate x 2 H2O"]
CultureMech_001160 --> ing5_Na3_citrate_x_2_H2O
CHEBI_32142["CHEBI:32142"]
ing5_Na3_citrate_x_2_H2O -.-> CHEBI_32142
ing6_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_001160 --> ing6_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing6_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing7_NaNO3["NaNO3"]
CultureMech_001160 --> ing7_NaNO3
CHEBI_63005["CHEBI:63005"]
ing7_NaNO3 -.-> CHEBI_63005
ing8_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_001160 --> ing8_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing9_NaHCO3["NaHCO3"]
CultureMech_001160 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Fe_NH4_citrate["Fe(NH4)citrate"]
CultureMech_001160 --> ing10_Fe_NH4_citrate
CHEBI_31604["CHEBI:31604"]
ing10_Fe_NH4_citrate -.-> CHEBI_31604
ing11_NH4Cl["NH4Cl"]
CultureMech_001160 --> ing11_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing11_NH4Cl -.-> CHEBI_31206
ing12_Vitamin_B12["Vitamin B12"]
CultureMech_001160 --> ing12_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing12_Vitamin_B12 -.-> CHEBI_176843
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001160 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001160 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_H3BO3["H3BO3"]
CultureMech_001160 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001160 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001160 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_001160 --> ing18_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing18_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
33.3 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
3.3 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 | Mg(SO4) x 7 H2O | 3.3 G_PER_L | ||
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Na3-citrate x 2 H2O
C6H5O7.2H2O.3Na 294.099 g/mol
|
0.00498 G_PER_L |
CHEBI:32142
sodium citrate dihydrate
|
|
| 7 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
0.00083 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 8 |
NaNO3
NO3.Na 84.994 g/mol
|
0.6225 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 9 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.0332 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.180075 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 | Fe(NH4)citrate | 0.0048 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 12 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.02675 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 13 | Vitamin B12 | 2e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.81 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
2.86 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.39 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
10 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.005 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Preparation
- Adjust to 1000 mL with H2O dist. and autoclave.
- After cooling, add the following filter sterilized components:
- Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- cyanobacteria_medium_ba_50_b_n_2 (CultureMech:001160)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.211329Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1678 |
| 2026-01-28T19:48:40.423052Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:01.767583Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001160 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.526334+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Cyanobacteria Medium BA+50+B+N/2" to "cyanobacteria_medium_ba_50_b_n_2" for consistent matching |
| 2026-06-05T07:40:55.091659+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.064843+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:29.420294+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 380380eece45fe3e4eb35c86d885eb93ecb66e2de0404dd2051eca388c327e97 |