Overview
- External ontology
- mediadive.medium:1689 (source link unavailable) — MEDIUM for SM250
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1689.pdf
Composition
flowchart LR
CultureMech_001171["medium_for_sm250"]
ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001171 --> ing0_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
ing1_NH4Cl["NH4Cl"]
CultureMech_001171 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KCl["KCl"]
CultureMech_001171 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001171 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_001171 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NaCl["NaCl"]
CultureMech_001171 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_NaHCO3["NaHCO3"]
CultureMech_001171 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_001171 --> ing7_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing7_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing8_NiCl2_x_2_H2O["NiCl2 x 2 H2O"]
CultureMech_001171 --> ing8_NiCl2_x_2_H2O
CHEBI_34887["CHEBI:34887"]
ing8_NiCl2_x_2_H2O -.-> CHEBI_34887
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001171 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001171 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001171 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_Na2SeO4["Na2SeO4"]
CultureMech_001171 --> ing12_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing12_Na2SeO4 -.-> CHEBI_77775
ing13_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001171 --> ing13_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing13_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001171 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_H3BO3["H3BO3"]
CultureMech_001171 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001171 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_Biotin["Biotin"]
CultureMech_001171 --> ing17_Biotin
CHEBI_15956["CHEBI:15956"]
ing17_Biotin -.-> CHEBI_15956
ing18_Folic_acid["Folic acid"]
CultureMech_001171 --> ing18_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing18_Folic_acid -.-> CHEBI_27470
ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001171 --> ing19_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing20_Riboflavine["Riboflavine"]
CultureMech_001171 --> ing20_Riboflavine
CHEBI_17015["CHEBI:17015"]
ing20_Riboflavine -.-> CHEBI_17015
ing21_Thiamine["Thiamine"]
CultureMech_001171 --> ing21_Thiamine
CHEBI_18385["CHEBI:18385"]
ing21_Thiamine -.-> CHEBI_18385
ing22_Nicotinic_acid["Nicotinic acid"]
CultureMech_001171 --> ing22_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing22_Nicotinic_acid -.-> CHEBI_15940
ing23_Pantothenic_acid["Pantothenic acid"]
CultureMech_001171 --> ing23_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing23_Pantothenic_acid -.-> CHEBI_7916
ing24_Cobalamin["Cobalamin"]
CultureMech_001171 --> ing24_Cobalamin
CHEBI_28911["CHEBI:28911"]
ing24_Cobalamin -.-> CHEBI_28911
ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001171 --> ing25_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
ing26_Lipoic_acid["Lipoic acid"]
CultureMech_001171 --> ing26_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing26_Lipoic_acid -.-> CHEBI_16494
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.33 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.033 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.165 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.784 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 9 |
NiCl2 x 2 H2O
Cl2Ni 129.599 g/mol
|
0.44999999999999996 G_PER_L |
CHEBI:34887
nickel dichloride
|
[Merged 2 duplicates: 0.284, 0.166] |
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.028 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.099 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 13 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.095 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 14 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.033 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 20 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 21 |
Riboflavine
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 22 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.05 G_PER_L |
CHEBI:18385
thiamine(1+)
|
|
| 23 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 24 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 25 |
Cobalamin
C62H88CoN13O14P 1329.37 g/mol
|
0.001 G_PER_L |
CHEBI:28911
cob(III)alamin
|
|
| 26 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 27 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Boil the medium for 5 min and cool on ice under N2 (100%). Do not bubble.
- pH Wert 7.6-8.0.
- Fill 10 ml in Hungate tubes with a rubber septum under N2 (100%) flux.
- Flush each Hungate tube with molecular hydrogen for 10 seconds.
- Autoclave at 121° for 15 min. After autoclaving add 0.01 ml of filter-sterilized vitamin solution per 10 ml media.
- Inject 3 ml of sterile air with sterile syringe into each Hungate tube before inoculation.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_for_sm250 (CultureMech:001171)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.245280Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1689 |
| 2026-01-28T19:48:41.049410Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:40.871583Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:01.872216Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001171 |
| 2026-03-17T06:30:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.640775+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM for SM250" to "medium_for_sm250" for consistent matching |
| 2026-06-05T07:41:29.311299+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.398602+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:06.132361+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:43.265289+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 01dcf0aaa9d6fb6b53f5386e13405368f0fbc6fe3cd0153208b65e02efcced41 |