Overview
- External ontology
- mediadive.medium:1699 (source link unavailable) — SULFURIMONAS CRATERIS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.5, 'max': 8.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1699.pdf
Composition
flowchart LR
CultureMech_001179["sulfurimonas_crateris_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001179 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_001179 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_NaCl["NaCl"]
CultureMech_001179 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001179 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_KCl["KCl"]
CultureMech_001179 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001179 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_NaNO3["NaNO3"]
CultureMech_001179 --> ing6_NaNO3
CHEBI_63005["CHEBI:63005"]
ing6_NaNO3 -.-> CHEBI_63005
ing7_Sulfur["Sulfur"]
CultureMech_001179 --> ing7_Sulfur
CHEBI_26833["CHEBI:26833"]
ing7_Sulfur -.-> CHEBI_26833
ing8_Na2CO3["Na2CO3"]
CultureMech_001179 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001179 --> ing9_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001179 --> ing10_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
ing11_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001179 --> ing11_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing11_MnSO4_x_H2O -.-> CHEBI_86364
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001179 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001179 --> ing13_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001179 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001179 --> ing15_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001179 --> ing16_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing17_H3BO3["H3BO3"]
CultureMech_001179 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001179 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001179 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001179 --> ing20_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001179 --> ing21_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing22_Biotin["Biotin"]
CultureMech_001179 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Folic_acid["Folic acid"]
CultureMech_001179 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001179 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Thiamine_HCl["Thiamine HCl"]
CultureMech_001179 --> ing25_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing25_Thiamine_HCl -.-> CHEBI_49105
ing26_Riboflavin["Riboflavin"]
CultureMech_001179 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_001179 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001179 --> ing28_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_Calcium_D_____pantothenate -.-> CHEBI_31345
ing29_Vitamin_B12["Vitamin B12"]
CultureMech_001179 --> ing29_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing29_Vitamin_B12 -.-> CHEBI_176843
CultureMech_001179 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.203874 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.254842 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
11.1937 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 10.1937, 1.0] |
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.407747 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.509684 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25290500000000005 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.152905, 0.1] |
| 7 |
NaNO3
NO3.Na 84.994 g/mol
|
0.866463 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 8 |
Sulfur
S 32.067 g/mol
|
5.09684 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
50 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 11 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 12 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 17 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 22 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 26 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 29 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 30 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 31 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 32 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve compounds of solution A except sulfur and sparge it with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials that contain the appropriate amount of sulfur and autoclave at <b>110°C</b> for 20 min. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas and sterilized by filtration. To complete the medium appropriate amounts of solutions B and C are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.5 - 8.0, if necessary.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sulfurimonas_crateris_medium (CultureMech:001179)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.279147Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1699 |
| 2026-02-06T05:10:42.629761Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:25.776643Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:01.957397Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001179 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.734881+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SULFURIMONAS CRATERIS MEDIUM" to "sulfurimonas_crateris_medium" for consistent matching |
| 2026-05-20T06:02:06.608353+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.5-8' -> {'min': 7.5, 'max': 8.0} |
| 2026-06-05T07:41:47.715861+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:12.125167+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.972713+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b795ba8c7002aa3619e265411e574a1bc31199170415723d8b8783acac9aa0e4 |