Overview
- External ontology
- mediadive.medium:1705 (source link unavailable) — AM5 MEDIUM (ELUSIMICROBIUM MEDIUM)
- Applications
-
- Microbial cultivation
- pH
- 7.2
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1705.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001186["am5_medium_elusimicrobium_medium"]
ing0_NaCl["NaCl"]
CultureMech_001186 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_001186 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001186 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001186 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_001186 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KH2PO4["KH2PO4"]
CultureMech_001186 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Resazurin["Resazurin"]
CultureMech_001186 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_NaHCO3["NaHCO3"]
CultureMech_001186 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_D_Glucose["D-Glucose"]
CultureMech_001186 --> ing8_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing8_D_Glucose -.-> CHEBI_17634
ing9_Cystein["Cystein"]
CultureMech_001186 --> ing9_Cystein
CHEBI_15356["CHEBI:15356"]
ing9_Cystein -.-> CHEBI_15356
ing10_Yeast_extract["Yeast extract"]
CultureMech_001186 --> ing10_Yeast_extract
ing11_Casamino_acids["Casamino acids"]
CultureMech_001186 --> ing11_Casamino_acids
ing12_Dithiothreitol["Dithiothreitol"]
CultureMech_001186 --> ing12_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing12_Dithiothreitol -.-> CHEBI_18320
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001186 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_ZnCl2["ZnCl2"]
CultureMech_001186 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001186 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001186 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001186 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001186 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001186 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_H3BO3["H3BO3"]
CultureMech_001186 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2_EDTA["Na2-EDTA"]
CultureMech_001186 --> ing21_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing21_Na2_EDTA -.-> CHEBI_64734
ing22_NaOH["NaOH"]
CultureMech_001186 --> ing22_NaOH
CHEBI_32145["CHEBI:32145"]
ing22_NaOH -.-> CHEBI_32145
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001186 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001186 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing25_Vitamin_B12["Vitamin B12"]
CultureMech_001186 --> ing25_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing25_Vitamin_B12 -.-> CHEBI_176843
ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001186 --> ing26_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
ing27_Biotin["Biotin"]
CultureMech_001186 --> ing27_Biotin
CHEBI_15956["CHEBI:15956"]
ing27_Biotin -.-> CHEBI_15956
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_001186 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001186 --> ing29_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_pantothenate -.-> CHEBI_31345
CultureMech_001186 --> more["...16 more"]
Ingredients (46)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0004 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
202.52021 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
[Merged 3 duplicates: 2.52021, 100.0, 100.0] |
| 9 |
D-Glucose
C6H12O6 180.156 g/mol
|
0.9008 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 10 |
Cystein
C3H7NO2S 121.161 g/mol
|
0.121159 G_PER_L |
CHEBI:15356
cysteine
|
|
| 11 | Yeast extract | 1 G_PER_L | ||
| 12 | Casamino acids | 0.2 G_PER_L | ||
| 13 |
Dithiothreitol
C4H10O2S2 154.256 g/mol
|
0.1543 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
2 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
5.2 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 23 |
NaOH
HO.Na 39.997 g/mol
|
300.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
[Merged 4 duplicates: 0.5, 100.0, 100.0, 100.0] |
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 26 | Vitamin B12 | 0.0027972 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 27 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.0027972 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 28 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.000559441 G_PER_L |
CHEBI:15956
biotin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.00559441 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.0013986 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.013986 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 32 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.0027972 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 33 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.0013986 G_PER_L |
CHEBI:27470
folic acid
|
|
| 34 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.0013986 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 35 |
Valeric acid
C5H10O2 102.133 g/mol
|
0.0727273 G_PER_L |
CHEBI:17418
valeric acid
|
|
| 36 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
0.0713287 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 37 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 38 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 39 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 40 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
0.0615385 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 41 |
Phenyl acetic acid
C8H8O2 136.15 g/mol
|
0.019021 G_PER_L |
CHEBI:30745
phenylacetic acid
|
|
| 42 |
Phenyl propionic acid
C9H10O2 150.177 g/mol
|
0.020979 G_PER_L |
CHEBI:28631
3-phenylpropionic acid
|
|
| 43 |
4-Hydroxyphenyl acetic acid
C8H8O3 152.149 g/mol
|
0.0212587 G_PER_L |
CHEBI:18101
4-hydroxyphenylacetic acid
|
|
| 44 |
3-Indolyl acetic acid
C10H9NO2 175.187 g/mol
|
0.0246154 G_PER_L |
CHEBI:16411
indole-3-acetic acid
|
|
| 45 |
Menadione
C11H8O2 172.183 g/mol
|
0.027972 G_PER_L |
CHEBI:28869
menadione
|
|
| 46 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.00559441 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components and autoclave. Cool the medium under a N2/CO2 (4:1, v/v) gas stream. Add anaerobically the following solutions:
- Adjust to pH 7.1-7.2 with Na2CO3 or HCl. Distribute the medium into culture vessels under the N2/CO2 (4:1, v/v) gas stream and seal with a butyl stopper.
- Filtrate if insoluble residues remain.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- am5_medium_elusimicrobium_medium (CultureMech:001186)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:15.297556Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1705 |
| 2026-01-28T19:48:15.033184Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:53.491175Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:02.021603Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001186 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.809574+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AM5 MEDIUM (ELUSIMICROBIUM MEDIUM)" to "am5_medium_elusimicrobium_medium" for consistent matching |
| 2026-06-05T07:40:48.372428+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 38 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.622080+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:52.978552+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.845414+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 681d0e80c540b3c12119e51ced7e55e6d4c38c245306e3863c21b379c6cd3dd7 |