lentimonas_medium

CultureMech:001187 bacterial DEFINED LIQUID
Original name: LENTIMONAS MEDIUM

Overview

External ontology
mediadive.medium:1706 (source link unavailable) — LENTIMONAS MEDIUM
Applications
  • Microbial cultivation
pH
7.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1706.pdf

Composition

flowchart LR
    CultureMech_001187["lentimonas_medium"]
    ing0_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001187 --> ing0_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing0_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing1_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001187 --> ing1_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing1_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing2_H3BO3["H3BO3"]
    CultureMech_001187 --> ing2_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing2_H3BO3 -.-> CHEBI_33118
    ing3_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001187 --> ing3_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing3_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing4_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001187 --> ing4_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing4_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing5_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001187 --> ing5_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing5_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing6_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001187 --> ing6_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing6_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing7_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001187 --> ing7_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing7_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing8_4_Aminobenzoic_Acid["4-Aminobenzoic Acid"]
    CultureMech_001187 --> ing8_4_Aminobenzoic_Acid
    CHEBI_30753["CHEBI:30753"]
    ing8_4_Aminobenzoic_Acid -.-> CHEBI_30753
    ing9_D_____biotin["D-(+)-biotin"]
    CultureMech_001187 --> ing9_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_D_____biotin -.-> CHEBI_15956
    ing10_Folic_acid["Folic acid"]
    CultureMech_001187 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Lipoic_acid["Lipoic acid"]
    CultureMech_001187 --> ing11_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing11_Lipoic_acid -.-> CHEBI_16494
    ing12_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001187 --> ing12_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing12_Nicotinic_acid -.-> CHEBI_15940
    ing13_Hemicalcium_D_____pantothenate["Hemicalcium D-(+)-pantothenate"]
    CultureMech_001187 --> ing13_Hemicalcium_D_____pantothenate
    ing14_Pyridoxamine_Dihydrochloride["Pyridoxamine Dihydrochloride"]
    CultureMech_001187 --> ing14_Pyridoxamine_Dihydrochloride
    CHEBI_131532["CHEBI:131532"]
    ing14_Pyridoxamine_Dihydrochloride -.-> CHEBI_131532
    ing15_Thiamine_hydrochloride["Thiamine hydrochloride"]
    CultureMech_001187 --> ing15_Thiamine_hydrochloride
    CHEBI_49105["CHEBI:49105"]
    ing15_Thiamine_hydrochloride -.-> CHEBI_49105
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_001187 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_NaCl["NaCl"]
    CultureMech_001187 --> ing17_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing17_NaCl -.-> CHEBI_26710
    ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001187 --> ing18_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing19_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001187 --> ing19_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing19_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing20_KCl["KCl"]
    CultureMech_001187 --> ing20_KCl
    CHEBI_32588["CHEBI:32588"]
    ing20_KCl -.-> CHEBI_32588
    ing21_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001187 --> ing21_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing21_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing22_Tris["Tris"]
    CultureMech_001187 --> ing22_Tris
    CHEBI_9754["CHEBI:9754"]
    ing22_Tris -.-> CHEBI_9754
    ing23_Na2_EDTA["Na2-EDTA"]
    CultureMech_001187 --> ing23_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing23_Na2_EDTA -.-> CHEBI_64734
    ing24_K2HPO4["K2HPO4"]
    CultureMech_001187 --> ing24_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing24_K2HPO4 -.-> CHEBI_131527
    ing25_KH2PO4["KH2PO4"]
    CultureMech_001187 --> ing25_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing25_KH2PO4 -.-> CHEBI_63036
    ing26_Yeast_extract["Yeast extract"]
    CultureMech_001187 --> ing26_Yeast_extract

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
2 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
3 H3BO3
H3BO3 61.833 g/mol
2.86 G_PER_L CHEBI:33118
boric acid
4 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.81 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
5 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.222 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
6 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.079 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
7 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0494 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
8 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.005 G_PER_L CHEBI:34887
nickel dichloride
9 4-Aminobenzoic Acid
C7H7NO2 137.138 g/mol
0.04 G_PER_L CHEBI:30753
4-aminobenzoic acid
10 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.01 G_PER_L CHEBI:15956
biotin
11 Folic acid
C19H19N7O6 441.404 g/mol
0.03 G_PER_L CHEBI:27470
folic acid
12 Lipoic acid
C8H14O2S2 206.332 g/mol
0.01 G_PER_L CHEBI:16494
lipoic acid
13 Nicotinic acid
C6H5NO2 123.111 g/mol
0.1 G_PER_L CHEBI:15940
nicotinic acid
14 Hemicalcium D-(+)-pantothenate 0.05 G_PER_L
15 Pyridoxamine Dihydrochloride
C8H12N2O2.2HCl 241.118 g/mol
0.11 G_PER_L CHEBI:131532
pyridoxamine dihydrochloride
16 Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
0.1 G_PER_L CHEBI:49105
thiamine hydrochloride
17 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
18 NaCl
Cl.Na 58.443 g/mol
49.9 G_PER_L CHEBI:26710
sodium chloride
19 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
20 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
21 KCl
Cl.K 74.551 g/mol
1.5 G_PER_L CHEBI:32588
potassium chloride
22 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.24 G_PER_L CHEBI:86158
calcium chloride dihydrate
23 Tris
C4H11NO3 121.136 g/mol
12 G_PER_L CHEBI:9754
tris
24 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.002 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
25 K2HPO4
HO4P.2K 174.174 g/mol
1.6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
26 KH2PO4
H2O4P.K 136.084 g/mol
0.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
27 Yeast extract 0.01 G_PER_L

Preparation

  1. For 1 l of medium combine 500 ml of solution I, 100 ml of solution II and 400 ml of solution III and add the following additives:
  2. Filtrate (0.22 µm).
  3. Filtrate (0.22 µm).
  4. Adjust pH to 7.8. Autoclave solutions to sterilize.
  5. Adjust pH to 7.8. Autoclave solutions to sterilize.
  6. Adjust pH to 7.8. Autoclave solutions to sterilize.

Solutions

Carbon source solution

Dose: 2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.304213Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1706
2026-03-09T08:04:50.650751+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:02.036842Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001187
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:30.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.827367+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LENTIMONAS MEDIUM" to "lentimonas_medium" for consistent matching
2026-05-16T09:03:38.997000+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:11.091639+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.525108+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:40.197405+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ad8ea8802a4d3aee614a95022e644d60cc48a48551ab69069f04e0e7bb5b0819