aromatoleum_medium

Overview

External ontology

mediadive.medium:1712 (source link unavailable) — AROMATOLEUM MEDIUM

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1712.pdf

Composition

flowchart LR
    CultureMech_001192["aromatoleum_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001192 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001192 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001192 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001192 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NaNO3["NaNO3"]
    CultureMech_001192 --> ing4_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing4_NaNO3 -.-> CHEBI_63005
    ing5_Na2_EDTA["Na2-EDTA"]
    CultureMech_001192 --> ing5_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing5_Na2_EDTA -.-> CHEBI_64734
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001192 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_H3BO3["H3BO3"]
    CultureMech_001192 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001192 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001192 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001192 --> ing10_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001192 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001192 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001192 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Na2HPO4_NaH2PO4_buffer["Na2HPO4-NaH2PO4 buffer"]
    CultureMech_001192 --> ing14_Na2HPO4_NaH2PO4_buffer
    ing15_4_Aminobenzoic_Acid["4-Aminobenzoic Acid"]
    CultureMech_001192 --> ing15_4_Aminobenzoic_Acid
    CHEBI_30753["CHEBI:30753"]
    ing15_4_Aminobenzoic_Acid -.-> CHEBI_30753
    ing16_D_____biotin["D-(+)-biotin"]
    CultureMech_001192 --> ing16_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing16_D_____biotin -.-> CHEBI_15956
    ing17_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001192 --> ing17_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing17_Nicotinic_acid -.-> CHEBI_15940
    ing18_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_001192 --> ing18_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing18_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing19_Pyridoxal_hydrochloride["Pyridoxal hydrochloride"]
    CultureMech_001192 --> ing19_Pyridoxal_hydrochloride
    CHEBI_131529["CHEBI:131529"]
    ing19_Pyridoxal_hydrochloride -.-> CHEBI_131529
    ing20_DL_alpha_lipoic_acid["DL-alpha-lipoic acid"]
    CultureMech_001192 --> ing20_DL_alpha_lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing20_DL_alpha_lipoic_acid -.-> CHEBI_16494
    ing21_Folic_acid["Folic acid"]
    CultureMech_001192 --> ing21_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing21_Folic_acid -.-> CHEBI_27470
    ing22_2_Mercaptoethanesulfonic_acid["2-Mercaptoethanesulfonic acid"]
    CultureMech_001192 --> ing22_2_Mercaptoethanesulfonic_acid
    CHEBI_17905["CHEBI:17905"]
    ing22_2_Mercaptoethanesulfonic_acid -.-> CHEBI_17905
    ing23_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_001192 --> ing23_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing23_Cyanocobalamine -.-> CHEBI_17439
    ing24_Double_distilled_water["Double distilled water"]
    CultureMech_001192 --> ing24_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing24_Double_distilled_water -.-> CHEBI_15377
    ing25_NaHCO3["NaHCO3"]
    CultureMech_001192 --> ing25_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing25_NaHCO3 -.-> CHEBI_32139
    ing26_Ascorbic_acid["Ascorbic acid"]
    CultureMech_001192 --> ing26_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing26_Ascorbic_acid -.-> CHEBI_22652
    ing27_Sodiumbenzoate["Sodiumbenzoate"]
    CultureMech_001192 --> ing27_Sodiumbenzoate
    CHEBI_113455["CHEBI:113455"]
    ing27_Sodiumbenzoate -.-> CHEBI_113455

Ingredients (28)

Preparation

1. The medium was autoclaved and removed from the autoclave at 85°C. Oxygen dissolution was prevented by exchanging the gas-phase with N2. The medium was complemented by addition of the following stock solutions:
2. After adding bicarbonate the gas was changed from N2 to N2/CO2 (90/10 %, v/v) and the pH was adjusted to 7.2 - 7.4 with sterile 2 M HCL.
3. The trace element solution was prepared by dissolving the salts in 800 mL warm H2Obidest
4. NaOH was used to adjust the pH to 6.5. The solution was added up to 1000 mL with H2Obidest and autoclaved.
5. The solution was filter-sterilized and stored at 4°C in the dark.
6. The solution was filter-sterilized and stored at 4°C in the dark.
7. The solution was dispensed in the required portions (40 mL per 1 L medium). The head space of the bottles was flushed with CO2. Saturation of CO2 in the solution was achieved by repeated flushing and shaking of the bottles. Finally, the bottles were sealed with rubber stoppers and aluminum crimps and autoclaved.
8. In an ice bath ascorbic acid was dissolved in 80 mL anoxic H2Obidest (autoclaved, cooled under N2 atmosphere) while flushing with N2. The pH was adjusted to 7.0 with NaOH and the solution added up to 100 ml with anoxic H2Obidest. After filter-sterilization the solution was stored under N2 atmosphere in a special flask (which can be flushed with gas) at 4°C in the dark.
9. The solution was filter-sterilized and stored at room temperature.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• aromatoleum_medium (CultureMech:001192)