Overview
- External ontology
- mediadive.medium:1717 (source link unavailable) — PERLABENTIMONAS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1717.pdf
Composition
flowchart LR
CultureMech_001195["perlabentimonas_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001195 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_001195 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KCl["KCl"]
CultureMech_001195 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_NaCl["NaCl"]
CultureMech_001195 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001195 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
CultureMech_001195 --> ing5_CaCl2_x_6_H2O
CHEBI_91243["CHEBI:91243"]
ing5_CaCl2_x_6_H2O -.-> CHEBI_91243
ing6_NaHCO3["NaHCO3"]
CultureMech_001195 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Yeast_extract["Yeast extract"]
CultureMech_001195 --> ing7_Yeast_extract
ing8_Tryptone["Tryptone"]
CultureMech_001195 --> ing8_Tryptone
ing9_Fructose["Fructose"]
CultureMech_001195 --> ing9_Fructose
CHEBI_28757["CHEBI:28757"]
ing9_Fructose -.-> CHEBI_28757
ing10_Resazurin["Resazurin"]
CultureMech_001195 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001195 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_Biotin["Biotin"]
CultureMech_001195 --> ing12_Biotin
CHEBI_15956["CHEBI:15956"]
ing12_Biotin -.-> CHEBI_15956
ing13_Folic_acid["Folic acid"]
CultureMech_001195 --> ing13_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing13_Folic_acid -.-> CHEBI_27470
ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001195 --> ing14_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing15_Thiamine_HCl["Thiamine HCl"]
CultureMech_001195 --> ing15_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing15_Thiamine_HCl -.-> CHEBI_49105
ing16_Riboflavin["Riboflavin"]
CultureMech_001195 --> ing16_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing16_Riboflavin -.-> CHEBI_17015
ing17_Nicotinic_acid["Nicotinic acid"]
CultureMech_001195 --> ing17_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing17_Nicotinic_acid -.-> CHEBI_15940
ing18_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001195 --> ing18_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing18_Calcium_D_____pantothenate -.-> CHEBI_31345
ing19_Vitamin_B12["Vitamin B12"]
CultureMech_001195 --> ing19_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing19_Vitamin_B12 -.-> CHEBI_176843
ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001195 --> ing20_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
ing21__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001195 --> ing21__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing21__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing22_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001195 --> ing22_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing22_Nitrilotriacetic_acid -.-> CHEBI_44557
ing23_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001195 --> ing23_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing23_MgSO4_x_7_H2O -.-> CHEBI_31795
ing24_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001195 --> ing24_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing24_MnSO4_x_H2O -.-> CHEBI_86364
ing25_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001195 --> ing25_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing25_FeSO4_x_7_H2O -.-> CHEBI_75836
ing26_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001195 --> ing26_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing26_CoSO4_x_7_H2O -.-> CHEBI_91244
ing27_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001195 --> ing27_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing27_CaCl2_x_2_H2O -.-> CHEBI_86158
ing28_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001195 --> ing28_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing28_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing29_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001195 --> ing29_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing29_CuSO4_x_5_H2O -.-> CHEBI_31440
CultureMech_001195 --> more["...6 more"]
Ingredients (36)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
11.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 10.0, 1.0] |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.33 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
|
0.033 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 | Yeast extract | 0.05 G_PER_L | ||
| 9 | Tryptone | 2 G_PER_L | ||
| 10 | Fructose | 2 G_PER_L |
CHEBI:28757
fructose
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 14 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 15 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 16 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 17 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 18 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 19 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 20 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 21 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 22 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 23 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 24 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 25 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 26 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 27 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 28 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 29 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 30 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 31 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 32 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 33 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 34 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 35 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 36 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients (except carbonate, and sulfide), adjust pH to 8.0, boil for 10 min. and sparge medium with 100% N2 gas for 30 min to make it anoxic. Distribute under N2 into Hungate tubes (10 ml) and autoclave. Add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Filter sterilize vitamin solution and aseptically add 100 µl per Hungate tube. Adjust pH of the complete medium to 8.0, if necessary.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- perlabentimonas_medium (CultureMech:001195)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.334851Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1717 |
| 2026-02-06T05:10:13.613066Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:40.189395Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:02.117029Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001195 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.921049+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PERLABENTIMONAS MEDIUM" to "perlabentimonas_medium" for consistent matching |
| 2026-06-05T07:41:41.353240+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.033664+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.601873+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7267de57d9088f127ad5a4951f7bca6ce2933afd74051bdaa7784c55f5a736c2 |