r2a_with_artificial_soda_salt_lake_water

CultureMech:001197 bacterial COMPLEX SOLID_AGAR
Original name: R2A WITH ARTIFICIAL SODA-SALT LAKE WATER

Overview

External ontology
mediadive.medium:1719 (source link unavailable) — R2A WITH ARTIFICIAL SODA-SALT LAKE WATER
Applications
  • Microbial cultivation
pH
9.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1719.pdf

Composition

flowchart LR
    CultureMech_001197["r2a_with_artificial_soda_salt_lake_water"]
    ing0_NaCl["NaCl"]
    CultureMech_001197 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Na2SO4["Na2SO4"]
    CultureMech_001197 --> ing1_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing1_Na2SO4 -.-> CHEBI_32149
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001197 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001197 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_001197 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_KBr["KBr"]
    CultureMech_001197 --> ing5_KBr
    CHEBI_32030["CHEBI:32030"]
    ing5_KBr -.-> CHEBI_32030
    ing6_H3BO3["H3BO3"]
    CultureMech_001197 --> ing6_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing6_H3BO3 -.-> CHEBI_33118
    ing7_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_001197 --> ing7_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing7_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing8_NaF["NaF"]
    CultureMech_001197 --> ing8_NaF
    CHEBI_28741["CHEBI:28741"]
    ing8_NaF -.-> CHEBI_28741
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_001197 --> ing9_Yeast_extract
    ing10_Proteose_peptone["Proteose peptone"]
    CultureMech_001197 --> ing10_Proteose_peptone
    ing11_Casamino_acids["Casamino acids"]
    CultureMech_001197 --> ing11_Casamino_acids
    ing12_Glucose["Glucose"]
    CultureMech_001197 --> ing12_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing12_Glucose -.-> CHEBI_17234
    ing13_Starch["Starch"]
    CultureMech_001197 --> ing13_Starch
    CHEBI_28017["CHEBI:28017"]
    ing13_Starch -.-> CHEBI_28017
    ing14_Na_pyruvate["Na-pyruvate"]
    CultureMech_001197 --> ing14_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing14_Na_pyruvate -.-> CHEBI_50144
    ing15_K2HPO4["K2HPO4"]
    CultureMech_001197 --> ing15_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing15_K2HPO4 -.-> CHEBI_131527
    ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001197 --> ing16_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing17_Agar["Agar"]
    CultureMech_001197 --> ing17_Agar
    CHEBI_2509["CHEBI:2509"]
    ing17_Agar -.-> CHEBI_2509

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
46.94 G_PER_L CHEBI:26710
sodium chloride
2 Na2SO4
2Na.O4S 142.043 g/mol
7.84 G_PER_L CHEBI:32149
sodium sulfate
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.24 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.17 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 KCl
Cl.K 74.551 g/mol
1.38 G_PER_L CHEBI:32588
potassium chloride
6 KBr
Br.K 119.002 g/mol
0.19 G_PER_L CHEBI:32030
potassium bromide
7 H3BO3
H3BO3 61.833 g/mol
0.05 G_PER_L CHEBI:33118
boric acid
8 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.08 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
9 NaF
F.Na 41.988 g/mol
0.06 G_PER_L CHEBI:28741
sodium fluoride
10 Yeast extract 0.5 G_PER_L
11 Proteose peptone 0.5 G_PER_L
12 Casamino acids 0.5 G_PER_L
13 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
14 Starch 0.5 G_PER_L CHEBI:28017
starch
15 Na-pyruvate
C3H3O3.Na 110.044 g/mol
0.3 G_PER_L CHEBI:50144
sodium pyruvate
16 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
17 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
18 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Add 3 g / L R2A (See medium 830).
  2. Autoclave to sterilize.
  3. Add aseptically 100 mL sterile Na2Co3 solution (50 g / L, autoclaved).
  4. pH 9.0.
  5. Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.345184Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1719
2026-01-28T19:48:21.262512Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:02.143099Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001197
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.950088+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R2A WITH ARTIFICIAL SODA-SALT LAKE WATER" to "r2a_with_artificial_soda_salt_lake_water" for consistent matching
2026-06-05T07:41:43.674268+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.047929+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:10.798225+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:54.168864+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 2809fd86be52e6000d923fd69e01067a3db4fbbf7a9e9a98a6b8eaaf76e9cbe1