mmjhs_medium

CultureMech:001200 bacterial DEFINED LIQUID
Original name: MMJHS MEDIUM

Overview

External ontology
mediadive.medium:1721 (source link unavailable) — MMJHS MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1721.pdf

Composition

flowchart LR
    CultureMech_001200["mmjhs_medium"]
    ing0_NaNO3["NaNO3"]
    CultureMech_001200 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_NaHCO3["NaHCO3"]
    CultureMech_001200 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001200 --> ing2_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing2_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing3_Sulfur["Sulfur"]
    CultureMech_001200 --> ing3_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing3_Sulfur -.-> CHEBI_26833
    ing4_NaCl["NaCl"]
    CultureMech_001200 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_K2HPO4["K2HPO4"]
    CultureMech_001200 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001200 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_NH4Cl["NH4Cl"]
    CultureMech_001200 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001200 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001200 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_KCl["KCl"]
    CultureMech_001200 --> ing10_KCl
    CHEBI_32588["CHEBI:32588"]
    ing10_KCl -.-> CHEBI_32588
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001200 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_001200 --> ing12_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001200 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001200 --> ing14_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001200 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001200 --> ing16_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing17_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_001200 --> ing17_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing17_AlK_SO4_2 -.-> CHEBI_86463
    ing18_H3BO3["H3BO3"]
    CultureMech_001200 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001200 --> ing19_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001200 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001200 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001200 --> ing22_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 NaNO3
NO3.Na 84.994 g/mol
1 G_PER_L CHEBI:63005
sodium nitrate
2 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
1 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
4 Sulfur
S 32.067 g/mol
10 G_PER_L CHEBI:26833
sulfur atom
5 NaCl
Cl.Na 58.443 g/mol
26.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 25.0, 1.0]
6 K2HPO4
HO4P.2K 174.174 g/mol
0.14 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.7999999999999999 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.7, 0.1]
8 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.4, 3.0]
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
12 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
13 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
16 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
17 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
18 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.002 G_PER_L CHEBI:86463
potassium aluminium sulfate
19 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
20 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.05 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding NaNO3, Na2S2O3 x 5 H20 and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, NaNO3, Na2S2O3 x 5 H20 and NaHCO3.
  2. Increase the 80% H2 + 20% CO2 gas phase pressure to 0.3 MPa.
  3. Adjust pH strain-specifically.
  4. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.351260Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1721
2026-03-13T03:09:03.396314Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:02.179453Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001200
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.984686+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MMJHS MEDIUM" to "mmjhs_medium" for consistent matching
2026-06-05T07:41:35.603752+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.325470+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:47.349088+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 4f2207a3f6ec36d2d4602b2da1140a357498acf176273cbe41b0f900bd441601