medium_for_thiorhodovibrio_frisius

CultureMech:001206 bacterial DEFINED LIQUID
Original name: MEDIUM FOR THIORHODOVIBRIO FRISIUS

Overview

External ontology
mediadive.medium:1729 (source link unavailable) — MEDIUM FOR THIORHODOVIBRIO FRISIUS
Applications
  • Microbial cultivation
pH
6.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1729.pdf

Composition

flowchart LR
    CultureMech_001206["medium_for_thiorhodovibrio_frisius"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001206 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001206 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KCl["KCl"]
    CultureMech_001206 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001206 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001206 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NaCl["NaCl"]
    CultureMech_001206 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001206 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_Sodium_acetate["Sodium acetate"]
    CultureMech_001206 --> ing7_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Sodium_acetate -.-> CHEBI_32954
    ing8_Double_distilled_water["Double distilled water"]
    CultureMech_001206 --> ing8_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Double_distilled_water -.-> CHEBI_15377
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001206 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_NaHCO3["NaHCO3"]
    CultureMech_001206 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Na2_EDTA["Na2-EDTA"]
    CultureMech_001206 --> ing11_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing11_Na2_EDTA -.-> CHEBI_64734
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001206 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001206 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
    CultureMech_001206 --> ing14_MnCl2_x_2_H2O
    CHEBI_131395["CHEBI:131395"]
    ing14_MnCl2_x_2_H2O -.-> CHEBI_131395
    ing15_ZnCl2["ZnCl2"]
    CultureMech_001206 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001206 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001206 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_H3BO3["H3BO3"]
    CultureMech_001206 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001206 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_Biotin["Biotin"]
    CultureMech_001206 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Folic_acid["Folic acid"]
    CultureMech_001206 --> ing21_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing21_Folic_acid -.-> CHEBI_27470
    ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001206 --> ing22_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001206 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Riboflavin["Riboflavin"]
    CultureMech_001206 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001206 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_001206 --> ing26_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_001206 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001206 --> ing28_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing29_Lipoic_acid["Lipoic acid"]
    CultureMech_001206 --> ing29_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing29_Lipoic_acid -.-> CHEBI_16494
    CultureMech_001206 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.25 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.34 G_PER_L CHEBI:31206
ammonium chloride
3 KCl
Cl.K 74.551 g/mol
0.34 G_PER_L CHEBI:32588
potassium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.8 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.25 G_PER_L CHEBI:32954
sodium acetate
9 Double distilled water
H2O 18.015 g/mol
1050.0 G_PER_L CHEBI:15377
water
 [Merged 2 duplicates: 1000.0, 50.0]
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
28.300227 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 [Merged 2 duplicates: 0.300227, 28.0]
11 NaHCO3
CHO3.Na 84.006 g/mol
30 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
3 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
0.05 G_PER_L CHEBI:131395
manganese(II) chloride dihydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.042 G_PER_L CHEBI:49976
zinc dichloride
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.018 G_PER_L CHEBI:75213
sodium molybdate dihydrate
19 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
20 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
21 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
22 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
23 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
24 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
25 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
26 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
27 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
28 Vitamin B12 0.021 G_PER_L CHEBI:176843
vitamin B12
 [Merged 2 duplicates: 0.001, 0.02]
29 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
30 Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
31 Na2CO3
CO3.2Na 105.988 g/mol
10.6 G_PER_L CHEBI:29377
sodium carbonate

Preparation

  1. Bubble with nitrogen gas and fill in tubes with a rubber septum under a stream of nitrogen gas.
  2. Autoclave at 121 °C for 15 min.
  3. aftre autoclaving add the following sterile solutions
  4. Adjust pH to 7.2.
  5. Add 1 ml/100ml of culture Sulfide feeding solution (Siefert and Pfennig 1984) every 3 days or when the sulfide is consumed.
  6. Fill 50 ml in 100 ml serum bottles, bubble for 15 min with CO2 and autoclave bottle capped tightly at 121°C for 15 min
  7. Adjust pH to 6.0.
  8. Filtrate (filter sterilze).
  9. Filtrate (filter sterilze).
  10. Filtrate (filter sterilze).
  11. Sparge medium with 100% CO2 gas mixture.
  12. Autoclave at 121 °C for 15 min.
  13. Sterile solution is saturated with CO2 (0.8 bar) until pH is approx. 7.3.
  14. 1 ml is added to 100 ml of culture approx. every 3 days or when the sulfide is consumed.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.371506Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1729
2026-03-13T03:08:52.735432Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:02.235493Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001206
2026-03-17T06:29:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.046050+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR THIORHODOVIBRIO FRISIUS" to "medium_for_thiorhodovibrio_frisius" for consistent matching
2026-06-05T07:41:30.117272+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.448082+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.153830+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 115563fb7ba56ac202fe672b6002a22c3c03fb1bb39c71094cecc43ef0ffb3be