beijerinckia_medium_lmg_18

Overview

External ontology

mediadive.medium:1736 (source link unavailable) — BEIJERINCKIA MEDIUM (LMG 18)

Applications

• Microbial cultivation

pH

6.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1736.pdf

Composition

flowchart LR
    CultureMech_001213["beijerinckia_medium_lmg_18"]
    ing0_Glucose["Glucose"]
    CultureMech_001213 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001213 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001213 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001213 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001213 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001213 --> ing5_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing5_MnSO4_x_H2O -.-> CHEBI_86364
    ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001213 --> ing6_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001213 --> ing7_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001213 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_Agar["Agar"]
    CultureMech_001213 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509

Ingredients (10)

Preparation

1. Final pH is 6.5.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Beijerinckia lactocogenes

  Strain

  NTCC 38849

  Evidence

  • Reference

    doi:10.1007/BF01999703

    Supports

    PARTIAL

    Explanation

    Brierley & Thornton 1979; Beijerinckia-type organism grown on a Becking pH 5 no-CaCO3 variant (see variant becking_medium_ph5_no_caco3). Medium-confidence: strain-to-run linkage is at the organism-set level.

    Snippet

    the same medium without CaCO3 adjusted to pH 5 was used for culturing of Beijerinckia-type organisms; B. lactocogenes NTCC 38849 was one of the organisms used; soil cultures incubated 21 days at 30C.

Recipe variants

• Name

  becking_medium_ph5_no_caco3

  Relationship

  DERIVED_FROM

  Description

  Becking medium variant for Beijerinckia-type organisms: CaCO3 omitted and pH adjusted to 5 (Brierley & Thornton 1979).

  Modifications

  • CaCO3 omitted relative to standard Becking medium.
  • pH adjusted to 5 (vs near-neutral parent).
  • Base recipe context: glucose 20 g/L, KH2PO4 1.0 g/L, MgSO4.7H2O 0.5 g/L (CaCO3 2.0 g/L before omission); soil cultures 21 d at 30C.

  Purpose

  Capture the acid, CaCO3-free Becking variant used for culturing Beijerinckia-type organisms.

  Evidence

  • Reference

    doi:10.1007/BF01999703

    Supports

    PARTIAL

    Explanation

    Brierley & Thornton 1979; explicit formulation change (no CaCO3, pH 5) for Beijerinckia culturing.

    Snippet

    the same medium without CaCO3 adjusted to pH 5 was used for culturing of Beijerinckia-type organisms.

Provenance

Merged from 1 source record.

• beijerinckia_medium_lmg_18 (CultureMech:001213)