methylomonas_medium_wsc

CultureMech:001215 bacterial DEFINED LIQUID
Original name: METHYLOMONAS MEDIUM (WSC)

Overview

External ontology
mediadive.medium:1738 (source link unavailable) — METHYLOMONAS MEDIUM (WSC)
Applications
  • Microbial cultivation
pH range
{'min': 7.8, 'max': 7.9}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1738.pdf

Composition

flowchart LR
    CultureMech_001215["methylomonas_medium_wsc"]
    ing0_NaCl["NaCl"]
    CultureMech_001215 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_001215 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001215 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaNO3["NaNO3"]
    CultureMech_001215 --> ing3_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing3_NaNO3 -.-> CHEBI_63005
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001215 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_H3BO3["H3BO3"]
    CultureMech_001215 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_001215 --> ing6_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing6_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing7_4_Aminobenzoic_Acid["4-Aminobenzoic Acid"]
    CultureMech_001215 --> ing7_4_Aminobenzoic_Acid
    CHEBI_30753["CHEBI:30753"]
    ing7_4_Aminobenzoic_Acid -.-> CHEBI_30753
    ing8_D_Biotin["D-Biotin"]
    CultureMech_001215 --> ing8_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_D_Biotin -.-> CHEBI_15956
    ing9_Folic_acid["Folic acid"]
    CultureMech_001215 --> ing9_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing9_Folic_acid -.-> CHEBI_27470
    ing10_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001215 --> ing10_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing10_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing11_Riboflavin["Riboflavin"]
    CultureMech_001215 --> ing11_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing11_Riboflavin -.-> CHEBI_17015
    ing12_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001215 --> ing12_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing12_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing13_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001215 --> ing13_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing13_Nicotinic_acid -.-> CHEBI_15940
    ing14_Calcium_D_Pantothenate["Calcium D-Pantothenate"]
    CultureMech_001215 --> ing14_Calcium_D_Pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing14_Calcium_D_Pantothenate -.-> CHEBI_31345
    ing15_Thioctic_acid["Thioctic acid"]
    CultureMech_001215 --> ing15_Thioctic_acid
    CHEBI_30314["CHEBI:30314"]
    ing15_Thioctic_acid -.-> CHEBI_30314
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_001215 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_HCl["HCl"]
    CultureMech_001215 --> ing17_HCl
    CHEBI_17883["CHEBI:17883"]
    ing17_HCl -.-> CHEBI_17883
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001215 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001215 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_CoCl2["CoCl2"]
    CultureMech_001215 --> ing20_CoCl2
    CHEBI_35696["CHEBI:35696"]
    ing20_CoCl2 -.-> CHEBI_35696
    ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001215 --> ing21_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001215 --> ing22_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001215 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_La_NO3_3_x_6_H2O["La(NO3)3 x 6 H2O"]
    CultureMech_001215 --> ing24_La_NO3_3_x_6_H2O
    ing25_Ce_NO3_3_x_6_H2O["Ce(NO3)3 x 6 H2O"]
    CultureMech_001215 --> ing25_Ce_NO3_3_x_6_H2O
    ing26_NaOH["NaOH"]
    CultureMech_001215 --> ing26_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing26_NaOH -.-> CHEBI_32145
    ing27_Na2SeO4["Na2SeO4"]
    CultureMech_001215 --> ing27_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing27_Na2SeO4 -.-> CHEBI_77775
    ing28_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001215 --> ing28_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing28_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing29_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001215 --> ing29_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing29_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    CultureMech_001215 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
5.26 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
2.24 G_PER_L CHEBI:32588
potassium chloride
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2.46 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 NaNO3
NO3.Na 84.994 g/mol
0.85 G_PER_L CHEBI:63005
sodium nitrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.88 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 H3BO3
H3BO3 61.833 g/mol
0.09 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.08, 0.01]
7 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.14 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
8 4-Aminobenzoic Acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
9 D-Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
10 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
11 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
12 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
13 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
14 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
15 Calcium D-Pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
16 Thioctic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:30314
(R)-lipoic acid
17 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
18 HCl
HCl 36.461 g/mol
3.646 G_PER_L CHEBI:17883
hydrogen chloride
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.09 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.07 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
21 CoCl2
2Cl.Co 129.839 g/mol
0.06 G_PER_L CHEBI:35696
cobalt dichloride
22 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.5 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
23 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.5 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
24 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.1 G_PER_L CHEBI:34887
nickel dichloride
25 La(NO3)3 x 6 H2O 0.06 G_PER_L
26 Ce(NO3)3 x 6 H2O 0.06 G_PER_L
27 NaOH
HO.Na 39.997 g/mol
12.200000000000001 G_PER_L CHEBI:32145
sodium hydroxide
 [Merged 2 duplicates: 0.4, 11.8]
28 Na2SeO4
2Na.O4Se 188.936 g/mol
0.11 G_PER_L CHEBI:77775
sodium selenate
29 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.05 G_PER_L CHEBI:63939
sodium tungstate dihydrate
30 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.29 G_PER_L CHEBI:75213
sodium molybdate dihydrate
31 HEPES 95.3 G_PER_L CHEBI:46756
HEPES
32 Na2HPO4
HO4P.2Na 141.958 g/mol
5.68 G_PER_L CHEBI:34683
disodium hydrogenphosphate

Preparation

  1. The growth medium contains the following components (per liter):
  2. Combine the distilled water and Solutions 1,3,4 in an Erlenmeyer flask and dispense into glass serum bottles.
  3. Flush the headspace of the serum bottles with pure N2. Seal the serum bottles with butyl rubber stoppers and crimp seals. Using a syringe and needle, inject pure methane and oxygen to final headspace concentrations of 5-10% CH4 and 21% O2.
  4. Autoclave the serum bottles for 20 minutes at 121 °C.
  5. Upon cooling to room temperature, add Solutions 2 and 5 from a filter-sterilized stock solution.
  6. The final pH of the medium should be 7.8 - 7.9.
  7. Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre
  8. Contains the following components per liter of deionized water:
  9. Adjust pH 7.8-7.9

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.396590Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1738
2026-03-13T03:09:31.065743Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:02.308943Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001215
2026-03-17T06:30:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.127673+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOMONAS MEDIUM (WSC)" to "methylomonas_medium_wsc" for consistent matching
2026-05-20T06:01:56.868182+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.8-7.9' -> {'min': 7.8, 'max': 7.9}
2026-06-05T07:41:33.239874+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.547001+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.599623+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.967486+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 6f65eec086acd804511df831848e0f44374223959f7a1a990442776965a92f2c