Overview
- External ontology
- mediadive.medium:173 (source link unavailable) — OCEANISPIROCHAETA LITORALIS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'spirochaeta_litoralis_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:173', 'original_category': 'bacterial'}
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium173.pdf
Composition
flowchart LR
CultureMech_001217["oceanispirochaeta_litoralis_medium"]
ing0_D_Glucose["D-Glucose"]
CultureMech_001217 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Trypticase_peptone["Trypticase peptone"]
CultureMech_001217 --> ing1_Trypticase_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_001217 --> ing2_Yeast_extract
ing3_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_001217 --> ing3_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing3_Tris_HCl_buffer -.-> CHEBI_9754
ing4_Sea_water["Sea water"]
CultureMech_001217 --> ing4_Sea_water
ing5_Sodium_resazurin["Sodium resazurin"]
CultureMech_001217 --> ing5_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Sodium_resazurin -.-> CHEBI_8806
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001217 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 2 | Trypticase peptone | 1 G_PER_L | ||
| 3 | Yeast extract | 1 G_PER_L | ||
| 4 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
50 G_PER_L |
CHEBI:9754
tris
|
|
| 5 | Sea water | 750 G_PER_L | ||
| 6 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Preparation
- Dissolve ingredients (except cysteine) and sparge medium for 30 - 45 min with 100% N2 gas to make it anoxic. Add cysteine and adjust pH to 7.2 - 7.4, then distribute medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Final pH of the medium should be about 7.2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- oceanispirochaeta_litoralis_medium (CultureMech:001217)
- spirochaeta_litoralis_medium (CultureMech:004202)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.405246Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 173 |
| 2026-03-13T06:19:02.332813Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001217 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.153028+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "OCEANISPIROCHAETA LITORALIS MEDIUM" to "oceanispirochaeta_litoralis_medium" for consistent matching |
| 2026-06-05T07:41:40.379858+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:51.912689+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: oceanispirochaeta_litoralis_medium, spirochaeta_litoralis_medium |
| 2026-08-06T03:05:51.912732+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 4efd972972fa2176093b4e8be0c07a877a787e20f8587f5d36608f541499bae6 |