Overview
- External ontology
- mediadive.medium:1740 (source link unavailable) — RHODOTHERMUS BIFROSTI MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1740.pdf
Composition
flowchart LR
CultureMech_001218["rhodothermus_bifrosti_medium"]
ing0_NaCl["NaCl"]
CultureMech_001218 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Yeast_extract["Yeast extract"]
CultureMech_001218 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_001218 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Peptone["Peptone"]
CultureMech_001218 --> ing3_Peptone
ing4_Tryptone_peptone["Tryptone peptone"]
CultureMech_001218 --> ing4_Tryptone_peptone
ing5_Glucose["Glucose"]
CultureMech_001218 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Starch["Starch"]
CultureMech_001218 --> ing6_Starch
CHEBI_28017["CHEBI:28017"]
ing6_Starch -.-> CHEBI_28017
ing7_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_001218 --> ing7_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing7_Sodium_pyruvate -.-> CHEBI_50144
ing8_Na2CO3["Na2CO3"]
CultureMech_001218 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Tap_water["Tap water"]
CultureMech_001218 --> ing9_Tap_water
CHEBI_15377["CHEBI:15377"]
ing9_Tap_water -.-> CHEBI_15377
ing10_Titriplex1["Titriplex1"]
CultureMech_001218 --> ing10_Titriplex1
ing11_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_001218 --> ing11_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing11_CaSO4_x_2_H2O -.-> CHEBI_32583
ing12_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001218 --> ing12_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing12_MgCl2_x_6_H2O -.-> CHEBI_86345
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001218 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001218 --> ing14_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
ing15_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001218 --> ing15_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing15_MnSO4_x_H2O -.-> CHEBI_86364
ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001218 --> ing16_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
ing17_CaCl2["CaCl2"]
CultureMech_001218 --> ing17_CaCl2
CHEBI_3312["CHEBI:3312"]
ing17_CaCl2 -.-> CHEBI_3312
ing18_CoCl2["CoCl2"]
CultureMech_001218 --> ing18_CoCl2
CHEBI_35696["CHEBI:35696"]
ing18_CoCl2 -.-> CHEBI_35696
ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001218 --> ing19_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001218 --> ing20_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
ing21_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001218 --> ing21_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing21_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing22_H3BO3["H3BO3"]
CultureMech_001218 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001218 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing24_Sodium_citrate["Sodium citrate"]
CultureMech_001218 --> ing24_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing24_Sodium_citrate -.-> CHEBI_53258
ing25_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_001218 --> ing25_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing25_FeCl3_x_6_H2O -.-> CHEBI_86254
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
11.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 10.0, 1.0] |
| 2 | Yeast extract | 1 G_PER_L | ||
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 | Peptone | 1 G_PER_L | ||
| 5 | Tryptone peptone | 1 G_PER_L | ||
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 7 | Starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
|
| 8 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.6 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.18 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
Tap water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
|
| 11 | Titriplex1 | 1.45055 G_PER_L | ||
| 12 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.43956 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 13 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.1978 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 16 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 17 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 18 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 19 |
CoCl2
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
|
| 20 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 21 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 22 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 25 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
2.94 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 26 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
2.7 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Preparation
- Adjust to pH 7.0. Autoclave at 121°C for 20 min.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals.
- Adjust to pH 7.0 with KOH
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- rhodothermus_bifrosti_medium (CultureMech:001218)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.406826Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1740 |
| 2026-03-13T03:09:30.529283Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:02.342149Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001218 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.162527+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RHODOTHERMUS BIFROSTI MEDIUM" to "rhodothermus_bifrosti_medium" for consistent matching |
| 2026-06-05T07:41:44.436998+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.999115+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.731497+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8f6b060a18a65c0c86ba5a3d312d912cb6b91cd4639d3bda3b1fe6ff0e3cbfd1 |