methylocystis_medium_nls

CultureMech:001219 bacterial DEFINED LIQUID
Original name: METHYLOCYSTIS MEDIUM (NLS)

Overview

External ontology
mediadive.medium:1741 (source link unavailable) — METHYLOCYSTIS MEDIUM (NLS)
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1741.pdf

Composition

flowchart LR
    CultureMech_001219["methylocystis_medium_nls"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001219 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_KNO3["KNO3"]
    CultureMech_001219 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001219 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_001219 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na2HPO4["Na2HPO4"]
    CultureMech_001219 --> ing4_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing4_Na2HPO4 -.-> CHEBI_34683
    ing5_NaOH["NaOH"]
    CultureMech_001219 --> ing5_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing5_NaOH -.-> CHEBI_32145
    ing6_Na2SeO4["Na2SeO4"]
    CultureMech_001219 --> ing6_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing6_Na2SeO4 -.-> CHEBI_77775
    ing7_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001219 --> ing7_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing7_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001219 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_4_Aminobenzoic_Acid["4-Aminobenzoic Acid"]
    CultureMech_001219 --> ing9_4_Aminobenzoic_Acid
    CHEBI_30753["CHEBI:30753"]
    ing9_4_Aminobenzoic_Acid -.-> CHEBI_30753
    ing10_D_Biotin["D-Biotin"]
    CultureMech_001219 --> ing10_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_D_Biotin -.-> CHEBI_15956
    ing11_Folic_acid["Folic acid"]
    CultureMech_001219 --> ing11_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing11_Folic_acid -.-> CHEBI_27470
    ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001219 --> ing12_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing13_Riboflavin["Riboflavin"]
    CultureMech_001219 --> ing13_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing13_Riboflavin -.-> CHEBI_17015
    ing14_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001219 --> ing14_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing14_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing15_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001219 --> ing15_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing15_Nicotinic_acid -.-> CHEBI_15940
    ing16_Calcium_D_Pantothenate["Calcium D-Pantothenate"]
    CultureMech_001219 --> ing16_Calcium_D_Pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_D_Pantothenate -.-> CHEBI_31345
    ing17_Thioctic_acid["Thioctic acid"]
    CultureMech_001219 --> ing17_Thioctic_acid
    CHEBI_30314["CHEBI:30314"]
    ing17_Thioctic_acid -.-> CHEBI_30314
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_001219 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843
    ing19_HCl["HCl"]
    CultureMech_001219 --> ing19_HCl
    CHEBI_17883["CHEBI:17883"]
    ing19_HCl -.-> CHEBI_17883
    ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001219 --> ing20_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001219 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_CoCl2["CoCl2"]
    CultureMech_001219 --> ing22_CoCl2
    CHEBI_35696["CHEBI:35696"]
    ing22_CoCl2 -.-> CHEBI_35696
    ing23_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001219 --> ing23_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing23_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing24_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001219 --> ing24_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing24_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001219 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_H3BO3["H3BO3"]
    CultureMech_001219 --> ing26_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing26_H3BO3 -.-> CHEBI_33118
    ing27_La_NO3_3_x_6_H2O["La(NO3)3 x 6 H2O"]
    CultureMech_001219 --> ing27_La_NO3_3_x_6_H2O
    ing28_Ce_NO3_3_x_6_H2O["Ce(NO3)3 x 6 H2O"]
    CultureMech_001219 --> ing28_Ce_NO3_3_x_6_H2O

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 KNO3
K.NO3 101.102 g/mol
20 G_PER_L CHEBI:63043
potassium nitrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.7 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 KH2PO4
H2O4P.K 136.084 g/mol
5.44 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 Na2HPO4
HO4P.2Na 141.958 g/mol
5.69 G_PER_L CHEBI:34683
disodium hydrogenphosphate
6 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
7 Na2SeO4
2Na.O4Se 188.936 g/mol
0.11 G_PER_L CHEBI:77775
sodium selenate
8 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.05 G_PER_L CHEBI:63939
sodium tungstate dihydrate
9 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.29 G_PER_L CHEBI:75213
sodium molybdate dihydrate
10 4-Aminobenzoic Acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
11 D-Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
12 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
13 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
14 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
15 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
16 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
17 Calcium D-Pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
18 Thioctic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:30314
(R)-lipoic acid
19 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
20 HCl
HCl 36.461 g/mol
3.646 G_PER_L CHEBI:17883
hydrogen chloride
21 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.09 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
22 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.07 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
23 CoCl2
2Cl.Co 129.839 g/mol
0.06 G_PER_L CHEBI:35696
cobalt dichloride
24 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.5 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
25 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.5 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
26 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.1 G_PER_L CHEBI:34887
nickel dichloride
27 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
28 La(NO3)3 x 6 H2O 0.06 G_PER_L
29 Ce(NO3)3 x 6 H2O 0.06 G_PER_L

Preparation

  1. The growth medium contains the following components (per liter):
  2. Bring up to 1 liter
  3. Combine the distilled water and Solutions A-D in an Erlenmeyer flask and dispense into glass serum bottles.
  4. Seal the serum bottles with butyl rubber stoppers and crimp seals. Using a syringe and needle, inject pure 10% methane.
  5. Autoclave the serum bottles for 20 minutes at 121 °C.
  6. Upon cooling to room temperature, add solution E from a filter-sterilized stock solution.
  7. The final pH of the medium should be 7.0
  8. Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre
  9. Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre
  10. Contains the following components per liter of distilled water:

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.411349Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1741
2026-03-13T06:19:02.355645Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001219
2026-03-17T06:30:16.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.176975+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOCYSTIS MEDIUM (NLS)" to "methylocystis_medium_nls" for consistent matching
2026-06-05T07:41:33.147362+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.529535+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.564302+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.786890+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint cf3d11dfdf5373ad37bd10937208f0738e0884d9293c023e18c112e052118e77