Overview
- External ontology
- mediadive.medium:1748 (source link unavailable) — HALOFERAX GIBBONSII MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1748.pdf
Composition
flowchart LR
CultureMech_001221["haloferax_gibbonsii_medium"]
ing0_Peptone["Peptone"]
CultureMech_001221 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001221 --> ing1_Yeast_extract
ing2_Tap_water["Tap water"]
CultureMech_001221 --> ing2_Tap_water
CHEBI_15377["CHEBI:15377"]
ing2_Tap_water -.-> CHEBI_15377
ing3_Agar["Agar"]
CultureMech_001221 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_NaCl["NaCl"]
CultureMech_001221 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_KCl["KCl"]
CultureMech_001221 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001221 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001221 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_CaCl2["CaCl2"]
CultureMech_001221 --> ing8_CaCl2
CHEBI_3312["CHEBI:3312"]
ing8_CaCl2 -.-> CHEBI_3312
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 10.0 G_PER_L | [Merged 2 duplicates: 5.0, 5.0] | |
| 2 | Yeast extract | 2.0 G_PER_L | [Merged 2 duplicates: 1.0, 1.0] | |
| 3 |
Tap water
H2O 18.015 g/mol
|
2000.0 G_PER_L |
CHEBI:15377
water
|
[Merged 2 duplicates: 1000.0, 1000.0] |
| 4 | Agar | 40.0 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) [Merged 2 duplicates: 20.0, 20.0] |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
240 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
7 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
30 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
35 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.5549 G_PER_L |
CHEBI:3312
calcium dichloride
|
Preparation
- or
- Adjust to pH 7.2 with 1 M Tris-Base and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.
- Adjust to pH 7.2 with 1 M Tris-Base and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.
- Adjust to pH 7.5 with 2 M Tris-Base and sterilize by autoclaving.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- haloferax_gibbonsii_medium (CultureMech:001221)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.422726Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1748 |
| 2026-03-13T03:09:41.118927Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:02.379019Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001221 |
| 2026-03-17T06:30:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.202092+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOFERAX GIBBONSII MEDIUM" to "haloferax_gibbonsii_medium" for consistent matching |
| 2026-06-05T07:41:07.776235+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:28.764034+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b951362597cbf1bfc49ba259bedae011169f4abda496154d49a9212a650ea906 |