Overview
- External ontology
- mediadive.medium:1755 (source link unavailable) — A2 MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1755.pdf
Composition
flowchart LR
CultureMech_001227["a2_medium"]
ing0_Sodium_acetate["Sodium acetate"]
CultureMech_001227 --> ing0_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing0_Sodium_acetate -.-> CHEBI_32954
ing1_Yeast_extract["Yeast extract"]
CultureMech_001227 --> ing1_Yeast_extract
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001227 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001227 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_K2HPO4["K2HPO4"]
CultureMech_001227 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KNO3["KNO3"]
CultureMech_001227 --> ing5_KNO3
CHEBI_63043["CHEBI:63043"]
ing5_KNO3 -.-> CHEBI_63043
ing6_HEPES["HEPES"]
CultureMech_001227 --> ing6_HEPES
CHEBI_46756["CHEBI:46756"]
ing6_HEPES -.-> CHEBI_46756
ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_001227 --> ing7_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
ing8_H3BO3["H3BO3"]
CultureMech_001227 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001227 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001227 --> ing10_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001227 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
CultureMech_001227 --> ing12_Na2_MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.24 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 2 | Yeast extract | 0.15 G_PER_L | ||
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.8 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.73 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.03, 3.7] |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.005 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
KNO3
K.NO3 101.102 g/mol
|
0.01 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 7 | HEPES | 4.766 G_PER_L |
CHEBI:46756
HEPES
|
|
| 8 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
2.5 G_PER_L |
CHEBI:33118
boric acid
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.87 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.005 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.44 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 13 |
Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.29 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.0-7.5
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- a2_medium (CultureMech:001227)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.434056Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1755 |
| 2026-03-13T03:09:14.557885Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:02.412977Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001227 |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.237835+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "A2 MEDIUM" to "a2_medium" for consistent matching |
| 2026-05-20T06:01:06.861758+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.5' -> {'min': 7.0, 'max': 7.5} |
| 2026-06-05T07:40:46.435712+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.451248+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:52.293126+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:21.118412+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 45fe81ec9153aed4f11642e801f4035f5f5d24ab36966490c86f8752ceb0642f |