cdmm

CultureMech:001228 bacterial COMPLEX LIQUID
Original name: CDMM

Overview

External ontology
mediadive.medium:1757 (source link unavailable) — CDMM
Applications
  • Microbial cultivation
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001228["cdmm"]
    ing0_Na2HPO4["Na2HPO4"]
    CultureMech_001228 --> ing0_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing0_Na2HPO4 -.-> CHEBI_34683
    ing1_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
    CultureMech_001228 --> ing1_NaH2PO4_x_H2O
    CHEBI_37585["CHEBI:37585"]
    ing1_NaH2PO4_x_H2O -.-> CHEBI_37585
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001228 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NaCl["NaCl"]
    CultureMech_001228 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Casamino_acids["Casamino acids"]
    CultureMech_001228 --> ing4_Casamino_acids
    ing5_L_Cysteine["L-Cysteine"]
    CultureMech_001228 --> ing5_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing5_L_Cysteine -.-> CHEBI_17561
    ing6_L_Tryptophan["L-Tryptophan"]
    CultureMech_001228 --> ing6_L_Tryptophan
    CHEBI_16828["CHEBI:16828"]
    ing6_L_Tryptophan -.-> CHEBI_16828
    ing7__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001228 --> ing7__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing7__NH4_2SO4 -.-> CHEBI_62946
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001228 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001228 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001228 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001228 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_NaHSeO3["NaHSeO3"]
    CultureMech_001228 --> ing12_NaHSeO3
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001228 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_D_Biotin["D-Biotin"]
    CultureMech_001228 --> ing14_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_D_Biotin -.-> CHEBI_15956
    ing15_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_001228 --> ing15_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing16_Pyridoxine["Pyridoxine"]
    CultureMech_001228 --> ing16_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing16_Pyridoxine -.-> CHEBI_16709
    ing17_alpha_D_Glucose["alpha-D-Glucose"]
    CultureMech_001228 --> ing17_alpha_D_Glucose
    CHEBI_17925["CHEBI:17925"]
    ing17_alpha_D_Glucose -.-> CHEBI_17925

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Na2HPO4
HO4P.2Na 141.958 g/mol
50 G_PER_L CHEBI:34683
disodium hydrogenphosphate
2 NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
20 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
9 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NaCl
Cl.Na 58.443 g/mol
9 G_PER_L CHEBI:26710
sodium chloride
5 Casamino acids 50 G_PER_L
6 L-Cysteine
C3H7NO2S 121.161 g/mol
2.5 G_PER_L CHEBI:17561
L-cysteine
7 L-Tryptophan
C11H12N2O2 204.229 g/mol
0.5 G_PER_L CHEBI:16828
L-tryptophan
8 (NH4)2SO4
2H4N.O4S 132.141 g/mol
4 G_PER_L CHEBI:62946
ammonium sulfate
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.1 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 NaHSeO3 0.015 G_PER_L (almost no effect on growth in CDMM, more important in MDM)
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 D-Biotin
C10H16N2O3S 244.316 g/mol
0.06 G_PER_L CHEBI:15956
biotin
16 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.2 G_PER_L CHEBI:31345
Calcium pantothenate
17 Pyridoxine
C8H11NO3 169.18 g/mol
0.2 G_PER_L CHEBI:16709
pyridoxine
18 alpha-D-Glucose
C6H12O6 180.156 g/mol
200 G_PER_L CHEBI:17925
alpha-D-glucose
 (not required but growth enhancing)

Preparation

  1. fill to 1 Liter with H2O (autoclaved)
  2. anaerobize with nitrogen, can be stored anaerobically
  3. autoclave
  4. pH should be between 6-7, may depend on the casamino acid and cysteine lot
  5. steril filtration, store as batches at -20°C
  6. steril filtration
  7. steril filtration, store in aliquots at -20°C
  8. steril filtration, store aliquots at -20°C
  9. steril filtration

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.436490Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1757
2026-01-28T19:48:15.971040Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:23.049943Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:02.422395Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001228
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.247657+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CDMM" to "cdmm" for consistent matching
2026-06-05T07:40:52.852822+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:36.226518+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:54.386911+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:27.011196+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 8cfc9a6ca526d6dae8da2fbd949e66c91b6bf727d94e48eea6365063a76462de