Overview
- External ontology
- mediadive.medium:1781 (source link unavailable) — MM3
- Applications
-
- Microbial cultivation
- pH range
- {'min': 5.8, 'max': 6.0}
- Notes
- Source: DSMZ
Composition
flowchart LR
CultureMech_001236["mm3"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001236 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_001236 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001236 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001236 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Yeast_extract["Yeast extract"]
CultureMech_001236 --> ing4_Yeast_extract
ing5_alpha_D_Glucose["alpha-D-Glucose"]
CultureMech_001236 --> ing5_alpha_D_Glucose
CHEBI_17925["CHEBI:17925"]
ing5_alpha_D_Glucose -.-> CHEBI_17925
ing6_Vitamin_B12["Vitamin B12"]
CultureMech_001236 --> ing6_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing6_Vitamin_B12 -.-> CHEBI_176843
ing7_4_aminobenzoate["4-aminobenzoate"]
CultureMech_001236 --> ing7_4_aminobenzoate
CHEBI_17836["CHEBI:17836"]
ing7_4_aminobenzoate -.-> CHEBI_17836
ing8_Biotin["Biotin"]
CultureMech_001236 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_Nicotinic_acid["Nicotinic acid"]
CultureMech_001236 --> ing9_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing9_Nicotinic_acid -.-> CHEBI_15940
ing10_Hemicalcium_D_____pantothenate["Hemicalcium D-(+)-pantothenate"]
CultureMech_001236 --> ing10_Hemicalcium_D_____pantothenate
ing11_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_001236 --> ing11_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing11_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing12_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001236 --> ing12_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing12_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing13_DL_6_8_thioctic_acid["DL-6,8-thioctic acid"]
CultureMech_001236 --> ing13_DL_6_8_thioctic_acid
CHEBI_16494["CHEBI:16494"]
ing13_DL_6_8_thioctic_acid -.-> CHEBI_16494
ing14_Riboflavin["Riboflavin"]
CultureMech_001236 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Folic_acid["Folic acid"]
CultureMech_001236 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_N_Acetylglucosamine["N-Acetylglucosamine"]
CultureMech_001236 --> ing16_N_Acetylglucosamine
CHEBI_59640["CHEBI:59640"]
ing16_N_Acetylglucosamine -.-> CHEBI_59640
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 | Yeast extract | 0.2 G_PER_L | ||
| 6 |
alpha-D-Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17925
alpha-D-glucose
|
|
| 7 | Vitamin B12 | 0.017 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 8 |
4-aminobenzoate
C7H6NO2 136.13 g/mol
|
0.013 G_PER_L |
CHEBI:17836
4-aminobenzoate
|
|
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.003 G_PER_L |
CHEBI:15956
biotin
|
|
| 10 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.033 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 11 | Hemicalcium D-(+)-pantothenate | 0.017 G_PER_L | ||
| 12 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.05 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 13 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.033 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 14 |
DL-6,8-thioctic acid
C8H14O2S2 206.332 g/mol
|
0.01 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.004 G_PER_L |
CHEBI:27470
folic acid
|
|
| 17 | N-Acetylglucosamine | 100 G_PER_L |
CHEBI:59640
N-acetylglucosamine
|
Preparation
- Filter sterilize the solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mm3 (CultureMech:001236)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.456350Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1781 |
| 2026-03-13T06:19:02.471218Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001236 |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.300948+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MM3" to "mm3" for consistent matching |
| 2026-05-20T06:01:58.131553+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5.8-6' -> {'min': 5.8, 'max': 6.0} |
| 2026-06-05T07:41:35.221426+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.228337+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.849229+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d8938603d0b64889f748fbf3e927aa7e2b5f4e215235b615442542bc1571e000 |