mucispirillum_spezial

Overview

External ontology

mediadive.medium:1790 (source link unavailable) — Mucispirillum Spezial

Applications

• Microbial cultivation

pH

7.0

Notes

Source: DSMZ Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification. Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001237["mucispirillum_spezial"]
    ing0_Calf_brains["Calf brains"]
    CultureMech_001237 --> ing0_Calf_brains
    ing1_Beef_heart["Beef heart"]
    CultureMech_001237 --> ing1_Beef_heart
    ing2_Proteose_peptone["Proteose peptone"]
    CultureMech_001237 --> ing2_Proteose_peptone
    ing3_Dextrose["Dextrose"]
    CultureMech_001237 --> ing3_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing3_Dextrose -.-> CHEBI_17634
    ing4_Sodium_chloride["Sodium chloride"]
    CultureMech_001237 --> ing4_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_chloride -.-> CHEBI_26710
    ing5_Disodium_phosphate["Disodium phosphate"]
    CultureMech_001237 --> ing5_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing5_Disodium_phosphate -.-> CHEBI_34683
    ing6_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_001237 --> ing6_Pancreatic_digest_of_casein
    ing7_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_001237 --> ing7_Peptic_digest_of_soybean_meal
    ing8_Glucose["Glucose"]
    CultureMech_001237 --> ing8_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing8_Glucose -.-> CHEBI_17234
    ing9_Sodium_chloride["Sodium chloride"]
    CultureMech_001237 --> ing9_Sodium_chloride
    ing9_Sodium_chloride -.-> CHEBI_26710
    ing10_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_001237 --> ing10_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing10_Dipotassium_phosphate -.-> CHEBI_131527
    ing11_Agar["Agar"]
    CultureMech_001237 --> ing11_Agar
    CHEBI_2509["CHEBI:2509"]
    ing11_Agar -.-> CHEBI_2509
    ing12_Yeast_extract["Yeast extract"]
    CultureMech_001237 --> ing12_Yeast_extract
    ing13_NaNO3["NaNO3"]
    CultureMech_001237 --> ing13_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing13_NaNO3 -.-> CHEBI_63005
    ing14_K2HPO4["K2HPO4"]
    CultureMech_001237 --> ing14_K2HPO4
    ing14_K2HPO4 -.-> CHEBI_131527
    ing15_Cysteine_HCl["Cysteine HCl"]
    CultureMech_001237 --> ing15_Cysteine_HCl
    ing16_Fetal_bovine_serum["Fetal bovine serum"]
    CultureMech_001237 --> ing16_Fetal_bovine_serum
    ing17_Haemin["Haemin"]
    CultureMech_001237 --> ing17_Haemin
    CHEBI_50385["CHEBI:50385"]
    ing17_Haemin -.-> CHEBI_50385
    ing18_NaOH["NaOH"]
    CultureMech_001237 --> ing18_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing18_NaOH -.-> CHEBI_32145
    ing19_Vitamin_K3["Vitamin K3"]
    CultureMech_001237 --> ing19_Vitamin_K3
    CHEBI_28869["CHEBI:28869"]
    ing19_Vitamin_K3 -.-> CHEBI_28869
    ing20_Ethanol["Ethanol"]
    CultureMech_001237 --> ing20_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing20_Ethanol -.-> CHEBI_16236

Ingredients (21)

Preparation

1. Adjust pH to 7.0.
2. Bring to boil, cool down under N2CO2.
3. Fill under N2CO2 in hungate tubes (10 ml)
4. Autoclave at 121 °C for 15 min.
5. pressurize with 1 bar H2
6. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.
7. Dissolve 5 mg vitamin K3 in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• mucispirillum_spezial (CultureMech:001237)