pas_for_acanthamoeba_hatchetti

CultureMech:001240 bacterial DEFINED LIQUID
Original name: PAS for Acanthamoeba hatchetti

Overview

External ontology
mediadive.medium:1796 (source link unavailable) — PAS for Acanthamoeba hatchetti
Applications
  • Microbial cultivation
pH
6.5
High-metal flag
True
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001240["pas_for_acanthamoeba_hatchetti"]
    ing0_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
    CultureMech_001240 --> ing0_Trisodium_citrate_x_2_H2O
    CHEBI_53258["CHEBI:53258"]
    ing0_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001240 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_Na2HPO4["Na2HPO4"]
    CultureMech_001240 --> ing2_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4 -.-> CHEBI_34683
    ing3_KH2PO4["KH2PO4"]
    CultureMech_001240 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001240 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
1 G_PER_L CHEBI:53258
sodium citrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
11.8 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 Na2HPO4
HO4P.2Na 141.958 g/mol
71 G_PER_L CHEBI:34683
disodium hydrogenphosphate
4 KH2PO4
H2O4P.K 136.084 g/mol
68.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
196.806 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate

Preparation

  1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
  2. Adjust pH to 7.0 with 1 N NaOH.
  3. Autoclave at 121 °C for 20 min.
  4. Store at room temperature.
  5. Make up to <span class='amount' data-volume='500'>500</span> ml with MilliQ water.
  6. Make up to <span class='amount' data-volume='500'>500</span> ml with MilliQ water.
  7. Make up to <span class='amount' data-volume='500'>500</span> ml with MilliQ water.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.465341Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1796
2026-01-28T19:48:16.045314Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:02.492538Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001240
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.323913+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PAS for Acanthamoeba hatchetti" to "pas_for_acanthamoeba_hatchetti" for consistent matching
2026-06-05T07:41:40.940697+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.900624+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:52.267521+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 4a774e44f0fa7ed5a27733e790b2f61b390c0a5b22b16164d0c5af284043f90c