Overview
- External ontology
- mediadive.medium:1797 (source link unavailable) — RE-103 Medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: DSMZ
Composition
flowchart LR
CultureMech_001241["re_103_medium"]
ing0_NaCl["NaCl"]
CultureMech_001241 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001241 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_KCl["KCl"]
CultureMech_001241 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001241 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Yeast_extract["Yeast extract"]
CultureMech_001241 --> ing4_Yeast_extract
ing5_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
CultureMech_001241 --> ing5_MnCl2_x_2_H2O
CHEBI_131395["CHEBI:131395"]
ing5_MnCl2_x_2_H2O -.-> CHEBI_131395
ing6_Agar["Agar"]
CultureMech_001241 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
206 G_PER_L |
CHEBI:26710
sodium chloride
|
(for Agar medium autoclave separately) |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
36 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.37 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 | Yeast extract | 5 G_PER_L | ||
| 6 |
MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
|
0.0075 G_PER_L |
CHEBI:131395
manganese(II) chloride dihydrate
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient |
Preparation
- Adjust pH to 7.0.
- Note: When preparing a solid medium, autoclave the NaCl separately.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- re_103_medium (CultureMech:001241)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.467237Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1797 |
| 2026-03-13T06:19:02.497187Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001241 |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.329026+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RE-103 Medium" to "re_103_medium" for consistent matching |
| 2026-06-05T07:41:44.052259+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:54.479061+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 492b26f7228bade3f087f0e8b7f885f1051611eef8cd947d343ef88d2f4275aa |