halohasta_a2

CultureMech:001266 archaea COMPLEX SOLID_AGAR
Original name: Halohasta (A2)

Overview

External ontology
mediadive.medium:1841 (source link unavailable) — Halohasta (A2)
Applications
  • Microbial cultivation
pH range
{'min': 7.5, 'max': 8.0}
High-metal flag
True
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001266["halohasta_a2"]
    ing0_NaCl["NaCl"]
    CultureMech_001266 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001266 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_KCl["KCl"]
    CultureMech_001266 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_KH2PO4["KH2PO4"]
    CultureMech_001266 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001266 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_001266 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_NaHCO3["NaHCO3"]
    CultureMech_001266 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_NaBr["NaBr"]
    CultureMech_001266 --> ing7_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing7_NaBr -.-> CHEBI_63004
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001266 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_001266 --> ing9_Yeast_extract
    ing10_Peptone__Oxoid_["Peptone (Oxoid)"]
    CultureMech_001266 --> ing10_Peptone__Oxoid_
    ing11_Agar["Agar"]
    CultureMech_001266 --> ing11_Agar
    CHEBI_2509["CHEBI:2509"]
    ing11_Agar -.-> CHEBI_2509
    ing12_Glucose["Glucose"]
    CultureMech_001266 --> ing12_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing12_Glucose -.-> CHEBI_17234
    ing13_Na_acetate["Na-acetate"]
    CultureMech_001266 --> ing13_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing13_Na_acetate -.-> CHEBI_32954
    ing14_Na_lactate["Na-lactate"]
    CultureMech_001266 --> ing14_Na_lactate
    CHEBI_75228["CHEBI:75228"]
    ing14_Na_lactate -.-> CHEBI_75228
    ing15_Na_butyrate["Na-butyrate"]
    CultureMech_001266 --> ing15_Na_butyrate
    CHEBI_64103["CHEBI:64103"]
    ing15_Na_butyrate -.-> CHEBI_64103
    ing16_HCl["HCl"]
    CultureMech_001266 --> ing16_HCl
    CHEBI_17883["CHEBI:17883"]
    ing16_HCl -.-> CHEBI_17883
    ing17_ZnCl2["ZnCl2"]
    CultureMech_001266 --> ing17_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing17_ZnCl2 -.-> CHEBI_49976
    ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001266 --> ing18_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing19_H3BO3["H3BO3"]
    CultureMech_001266 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001266 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001266 --> ing21_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001266 --> ing22_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001266 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_Biotin["Biotin"]
    CultureMech_001266 --> ing24_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing24_Biotin -.-> CHEBI_15956
    ing25_Folic_acid["Folic acid"]
    CultureMech_001266 --> ing25_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing25_Folic_acid -.-> CHEBI_27470
    ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001266 --> ing26_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing27_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001266 --> ing27_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing27_Thiamine_HCl -.-> CHEBI_49105
    ing28_Riboflavin["Riboflavin"]
    CultureMech_001266 --> ing28_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing28_Riboflavin -.-> CHEBI_17015
    ing29_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001266 --> ing29_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing29_Nicotinic_acid -.-> CHEBI_15940
    CultureMech_001266 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
50 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
4 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.36 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
7 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 NaBr
Br.Na 102.894 g/mol
0.23 G_PER_L CHEBI:63004
sodium bromide
9 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
161.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
 [Merged 2 duplicates: 160.0, 1.5]
10 Yeast extract 1 G_PER_L
11 Peptone (Oxoid) 5 G_PER_L
12 Agar 20 G_PER_L CHEBI:2509
agar
 Optional ingredient
13 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
14 Na-acetate
C2H3O2.Na 82.034 g/mol
10 G_PER_L CHEBI:32954
sodium acetate
15 Na-lactate
C3H5O3.Na 112.06 g/mol
10 G_PER_L CHEBI:75228
sodium lactate
16 Na-butyrate
C4H7O2.Na 110.088 g/mol
10 G_PER_L CHEBI:64103
sodium butyrate
17 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
18 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
19 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
20 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
21 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
22 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
23 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
25 Biotin
C10H16N2O3S 244.316 g/mol
0.2 G_PER_L CHEBI:15956
biotin
26 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
27 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
28 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
29 Riboflavin
C17H20N4O6 376.369 g/mol
0.1 G_PER_L CHEBI:17015
riboflavin
30 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
31 DL-Pantothenic acid
C9H17NO5 219.237 g/mol
0.05 G_PER_L CHEBI:7916
pantothenic acid
32 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
33 Para-aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid

Preparation

  1. Adjust pH to 7.5-8.0.
  2. after autoklaving add the following filter sterilied solutions.
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
  4. Filter sterilise.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.529387Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1841
2026-03-13T03:09:13.071473Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:02.683535Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001266
2026-03-17T06:30:20.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.531583+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Halohasta (A2)" to "halohasta_a2" for consistent matching
2026-05-20T06:01:22.066248+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.5-8' -> {'min': 7.5, 'max': 8.0}
2026-06-05T07:41:07.808137+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.526174+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:28.794023+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint fcad52e95a3831188b5c38c2ea04d7cb077e1adee31412f21c3e44289c5d43da