rhizobium_medium_for_isolation

CultureMech:001270 bacterial COMPLEX SOLID_AGAR
Original name: Rhizobium medium for isolation

Overview

External ontology
mediadive.medium:1848 (source link unavailable) — Rhizobium medium for isolation
Applications
  • Microbial cultivation
pH range
{'min': 6.6, 'max': 7.0}
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001270["rhizobium_medium_for_isolation"]
    ing0_D_Mannitol["D-Mannitol"]
    CultureMech_001270 --> ing0_D_Mannitol
    CHEBI_16899["CHEBI:16899"]
    ing0_D_Mannitol -.-> CHEBI_16899
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001270 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgSO4["MgSO4"]
    CultureMech_001270 --> ing2_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing2_MgSO4 -.-> CHEBI_32599
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_001270 --> ing3_Yeast_extract
    ing4_NaCl["NaCl"]
    CultureMech_001270 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Agar["Agar"]
    CultureMech_001270 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 D-Mannitol
C6H14O6 182.172 g/mol
10 G_PER_L CHEBI:16899
D-mannitol
2 K2HPO4
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MgSO4
Mg.O4S 120.368 g/mol
0.2 G_PER_L CHEBI:32599
magnesium sulfate
4 Yeast extract 1 G_PER_L
5 NaCl
Cl.Na 58.443 g/mol
0.1 G_PER_L CHEBI:26710
sodium chloride
6 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with distilled water.
  2. Heat to boiling to dissolve the medium completely
  3. Autoclave at 121 °C for 15 min.
  4. Cool down to 45-50°C.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.547081Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1848
2026-03-13T06:19:02.719827Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001270
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.569551+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Rhizobium medium for isolation" to "rhizobium_medium_for_isolation" for consistent matching
2026-05-20T06:02:04.061372+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.6-7' -> {'min': 6.6, 'max': 7.0}
2026-06-05T07:41:44.213976+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.911104+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:54.619500+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 2fc65d21ac0c2ae91307b0188aab54e1d40f020cd8746ace3ade0dec28befb30