Overview
- External ontology
- mediadive.medium:185 (source link unavailable) — THERMOPROTEUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium185.pdf
Composition
flowchart LR
CultureMech_001275["thermoproteus_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_001275 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001275 --> ing1_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing1_FeSO4_x_7_H2O -.-> CHEBI_75836
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001275 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_001275 --> ing3_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing3_CaSO4_x_2_H2O -.-> CHEBI_32583
ing4_KH2PO4["KH2PO4"]
CultureMech_001275 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Resazurin["Resazurin"]
CultureMech_001275 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Yeast_extract["Yeast extract"]
CultureMech_001275 --> ing6_Yeast_extract
ing7_Starch["Starch"]
CultureMech_001275 --> ing7_Starch
CHEBI_28017["CHEBI:28017"]
ing7_Starch -.-> CHEBI_28017
ing8_Sulfur["Sulfur"]
CultureMech_001275 --> ing8_Sulfur
CHEBI_26833["CHEBI:26833"]
ing8_Sulfur -.-> CHEBI_26833
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001275 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_NaF["NaF"]
CultureMech_001275 --> ing10_NaF
CHEBI_28741["CHEBI:28741"]
ing10_NaF -.-> CHEBI_28741
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001275 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_001275 --> ing12_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing12_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001275 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001275 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001275 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001275 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.264 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.556 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.492 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.344 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.014 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 | Yeast extract | 0.2 G_PER_L | ||
| 8 | Starch | 5 G_PER_L |
CHEBI:28017
starch
|
|
| 9 |
Sulfur
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
NaF
F.Na 41.988 g/mol
|
0.84 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.18 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.45 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.005 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.003 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.001 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Preparation
- Prepare medium (without starch, sodium sulfide, sulfur) anaerobically under N2 gas atmosphere. Adjust pH to 5.5 with H2SO4 before sterilization. Distribute the medium into tubes containing the appropriate amount of sulfur powder. Sterilize medium by heating for 3 h at 90 - 100°C on three subsequent days. Before use, add to the medium starch and sodium sulfide.
- Medium pH is 5.5.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- thermoproteus_medium (CultureMech:001275)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.414912Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 185 |
| 2026-03-13T06:19:02.741951Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001275 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.593390+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THERMOPROTEUS MEDIUM" to "thermoproteus_medium" for consistent matching |
| 2026-06-05T07:41:50.442269+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:13.125698+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:33.000646+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ec4b468348f9352d678c99ecf2709d46e96fe8e2ce3a240d6ac022e63ba5ec5a |