Overview
- External ontology
- mediadive.medium:1874 (source link unavailable) — 70:30 sporulation medium
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001277["70_30_sporulation_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_001277 --> ing0_Bacto_peptone
ing1_Proteose_peptone["Proteose peptone"]
CultureMech_001277 --> ing1_Proteose_peptone
ing2_Ammonium_Sulfate["Ammonium Sulfate"]
CultureMech_001277 --> ing2_Ammonium_Sulfate
CHEBI_62946["CHEBI:62946"]
ing2_Ammonium_Sulfate -.-> CHEBI_62946
ing3_Tris_base["Tris base"]
CultureMech_001277 --> ing3_Tris_base
CHEBI_9754["CHEBI:9754"]
ing3_Tris_base -.-> CHEBI_9754
ing4_Calf_brains["Calf brains"]
CultureMech_001277 --> ing4_Calf_brains
ing5_Beef_heart["Beef heart"]
CultureMech_001277 --> ing5_Beef_heart
ing6_Proteose_peptone["Proteose peptone"]
CultureMech_001277 --> ing6_Proteose_peptone
ing7_Dextrose["Dextrose"]
CultureMech_001277 --> ing7_Dextrose
CHEBI_17634["CHEBI:17634"]
ing7_Dextrose -.-> CHEBI_17634
ing8_Sodium_chloride["Sodium chloride"]
CultureMech_001277 --> ing8_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing8_Sodium_chloride -.-> CHEBI_26710
ing9_Disodium_phosphate["Disodium phosphate"]
CultureMech_001277 --> ing9_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing9_Disodium_phosphate -.-> CHEBI_34683
ing10_Yeast_extract["Yeast extract"]
CultureMech_001277 --> ing10_Yeast_extract
ing11_Agar["Agar"]
CultureMech_001277 --> ing11_Agar
CHEBI_2509["CHEBI:2509"]
ing11_Agar -.-> CHEBI_2509
ing12_L_Cysteine["L-Cysteine"]
CultureMech_001277 --> ing12_L_Cysteine
CHEBI_17561["CHEBI:17561"]
ing12_L_Cysteine -.-> CHEBI_17561
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 63 G_PER_L | ||
| 2 | Proteose peptone | 3.5 G_PER_L | ||
| 3 |
Ammonium Sulfate
2H4N.O4S 132.141 g/mol
|
0.7 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
Tris base
C4H11NO3 121.136 g/mol
|
1.06 G_PER_L |
CHEBI:9754
tris
|
|
| 5 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 6 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 7 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 8 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 9 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 10 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 11 | Yeast extract | 1.5 G_PER_L | ||
| 12 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 13 |
L-Cysteine
C3H7NO2S 121.161 g/mol
|
100 G_PER_L |
CHEBI:17561
L-cysteine
|
Preparation
- Autoclave at 121 °C for 15 min.
- Add cysteine solution after cooling to approximately 65°C.
- Store plates at 4°C for up to 1 week.
- Filtrate (pore size 0.22 µm).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- 70_30_sporulation_medium (CultureMech:001277)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.565440Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1874 |
| 2026-03-13T06:19:02.764652Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001277 |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.617560+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "70:30 sporulation medium" to "70_30_sporulation_medium" for consistent matching |
| 2026-06-05T07:40:15.895336+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:34.193305+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f15e228388e94a9331203cf5325e44125bf32bda627ab256141ff18d026822a3 |