desulfocapsa_sulfexigens_medium

Overview

External ontology

mediadive.medium:196 (source link unavailable) — DESULFOCAPSA SULFEXIGENS MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'desulfobulbus_sp_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:196', 'original_category': 'bacterial'}
• {'name': 'medium_196_modified_for_dsm_11383', 'source': 'KOMODO', 'source_id': 'komodo.medium:196_11383', 'original_category': 'bacterial'}
• {'name': 'medium_196_modified_for_dsm_13871', 'source': 'KOMODO', 'source_id': 'komodo.medium:196_13871', 'original_category': 'bacterial'}
• {'name': 'medium_196_modified_for_dsm_9700', 'source': 'KOMODO', 'source_id': 'komodo.medium:196_9700', 'original_category': 'bacterial'}

pH

7.2

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium196.pdf

Composition

flowchart LR
    CultureMech_001295["desulfocapsa_sulfexigens_medium"]
    ing0_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001295 --> ing0_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing0_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing1_Na2SO4["Na2SO4"]
    CultureMech_001295 --> ing1_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing1_Na2SO4 -.-> CHEBI_32149
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001295 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_001295 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_NaCl["NaCl"]
    CultureMech_001295 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001295 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_KCl["KCl"]
    CultureMech_001295 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001295 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_Na2CO3["Na2CO3"]
    CultureMech_001295 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001295 --> ing9_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing9_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing10_HCl["HCl"]
    CultureMech_001295 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001295 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_001295 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001295 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_001295 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001295 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001295 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001295 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001295 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (19)

Preparation

1. First dissolve 2.7 g FeCl3 x 6 H2O in distilled water and adjust the pH to 7.0 with 1 N NaOH (about 3 ml). Add and dissolve remaining salts of solution A and sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 min, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is autoclaved separately under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B and C are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be adjusted to 7.2.
2. Note: For transfers use 5 - 10% (v/v) inoculum.
3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 5 source records.

• desulfobulbus_sp_medium (CultureMech:004257)
• desulfocapsa_sulfexigens_medium (CultureMech:001295)
• medium_196_modified_for_dsm_11383 (CultureMech:004254)
• medium_196_modified_for_dsm_13871 (CultureMech:004255)
• medium_196_modified_for_dsm_9700 (CultureMech:004256)